灭幼脲对4种真菌菌丝生长的影响

Effects of Diflubenzuron on the Growth of Four Fungal Mycelia

研究了杀灭鳞翅目类害虫的农药灭幼脲对镰刀菌(Fusarium sp.)、疣孢漆斑霉(Myrothecium verrucaria)、粉红黏帚菌(Gliocladium roseum)和蛹虫草(Cordyceps militaris)4种真菌菌丝生长的影响。将灭幼脲按一定的浓度梯度加入到PDA培养基中,接菌后采用十字交叉法每7 d测量1次菌落直径,并计算抑制率,当空白组长满培养皿时终止测量。试验结果表明:灭幼脲对4种真菌菌丝生长有明显的抑制作用,且不同浓度的灭幼脲对不同真菌菌丝的生长影响程度不同。当灭幼脲浓度为800 mg/L时,对镰刀菌和疣孢漆斑霉的抑制率均为100%;对粉红黏帚菌的抑制率为95.24%;对蛹虫草的抑制率为99.49%。由此表明灭幼脲不仅能杀灭鳞翅目类的害虫,且对真菌菌丝的生长也起到一定的抑制作用。

This paper studies the effects of pesticide diflubenzuron, which kills lepidopteran pests, in inhibiting the growth of several fungi mycelia： Fusarium sp., Myrothecium verrucaria, Gliocladium roseum and Cordyceps militaris. Diflubenzuron were added according to certain concentration gradient into PDA medium. The crossing method was adopted every 7 days to measure several fungal colony diameter, and the inhibitory rate was calculated after inoculation until the plate in the control group is full of colonies.The results showed that diflubenzuron had obvious inhibitory effect on several kinds of fungal mycelia growth, and there are different influence with different concentrations of diflubenzuron on different fungal mycelia growth. When the concentration of diflubenzuron was 800 ppm, the inhibition rate on Fusarium sp. was 100%; the inhibitory rate on Gliocladium roseum was 95.24% ; the inhibitory rate on Myrothecium verrucaria was 100% ; the inhibitory rate on Cordyceps militaris was 99.49%. The results show that diflubenzuron can not only kill Lepidoptera pests, but also inhibit the mycelia growth.