三维纳米枝状物表面制备蛋白质印迹聚合物及其性能

Preparation and Properties of Hemoglobin Imprinted Polymers on Three-Dimensional Nanodendrites

在金电极表面制备了3D金/铜纳米枝状物,并以丙烯酰胺为功能单体,N,N-亚甲基双丙烯酰胺为交联剂,血红蛋白为模板分子,在枝状物表面成功制备了蛋白质印迹聚合物。利用循环伏安法和扫描电子显微镜对制备的聚合物修饰电极进行了表征,通过微分脉冲伏安法、利用制备的聚合物修饰电极可对溶液中的血红蛋白进行检测,得到比已报道的类似印迹传感器更宽的检测范围和更低的检测下限,其线性范围为1.0×10^(-14)~1.0×10^(-1)mg/L,检测限为1.9×10^(-15)mg/L(S/N=3),相关系数为0.9975。实验结果表明,在电极表面制备的金/铜纳米枝状物,大大提高了电极的电子传递能力,也提高了印迹聚合物修饰电极的检测范围。

For sensitive bioassay of hemoglobin （ Hb）, we synthesized Hb imprinted polymers （PIPs） on the surface of 3D Au/Cu nanodendrites prepared on Au electrode. The 3D Au/Cu nanodendrites were fabricated by electrodeposition performed by chronoamperometry at -0. 9 V （vs. SCE） for 400 s in an aqueous electrolyte containing CuSO4.5H20 （0. 1 mol/L） and HAuC14.4H20 （0.025 mol/L）. The PIPs was prepared with acrylamide as functional monomer, N,N＇-methylenebisacrylamide and Hb as crosslinking agent and the template, respectively. The electrode modified with PIPs was examined by cyclic voltammetry （CV） and scanning electron microscope （SEM）. Assays of Hb by differential pulse voltammetry （DPV） with PIPs modified electrode show broader linear range and lower detection limit for Hb determination compared to those similar sensors based on PIPs. The linear range is 1.0 × 10^-14 - 1.0 × 10^-1 mg/L with a detection limit at 1.9 × 10^-15 mg/L（ S/N = 3 ）. These results indicate that the 3D Au/Cu nanodendrites greatly improve electron transfer ability of electrodes, and also improve the detection range of electrodes modified with PIPs.