拟南芥表皮毛发育突变体abt3-1的基因定位及表型分析

Gene Mapping and Phenotype Analyses of an Arabidopsis thaliana Trichome Developmental Mutant abt3-1

在发掘和鉴定调控植物表皮毛发育的新因子过程中,获得了一个表皮毛发育异常的拟南芥隐性突变体abt3-1(aberrantly branched trichome 3-1)。与野生型拟南芥(Col-0)相比,其表皮毛分支数目明显增加。另外,abt3-1还表现出植株小、叶形宽、叶色发灰、主根短等发育缺陷。利用图位克隆技术将该突变基因ABT3定位在1号染色体上,分子标记在F28G11#3与F4N21#1之间,物理距离为134kb。该研究将为进一步克隆ABT3基因及研究其在调控植物生长发育过程中的作用奠定基础。

In the process of discovering and identifying new factors that regulate the development of plant trichome,we obtained a recessive Arabidopsis thaliana mutant aberrantly branched trichome 3-1（abt3-1）.Compared to the wild type（Col-0）A.thaliana,abt3-1appeared a high proportion of multi-branched trichomes.In addition,abt3-1is dramatically reduced in size and has a defective primary root,wide shaped grey leaves.The mutant gene ABT3 was precisely mapped into the 134 kb chromosome interval between the molecular markers F28G11#3and F4N21#1on chromosome 1,which was determined using the position cloning strategy.This research will lay a foundation for further cloning ABT3 gene and studying its role in the regulation of plant growth and development.