鸡骨草冷浸提取物抗病毒活性研究

Antiviral Activity of Cold-soak Extraction of Abrus Cantoniensis Hance

目的研究中药鸡骨草冷浸提取物的抗病毒活性。方法采用细胞体外抗病毒实验法,结合CPE法及CCK-8试剂盒,以TI值(治疗指数)为研究指标,以利巴韦林、阿昔洛韦为阳性对照药,研究鸡骨草冷浸提取物对呼吸道合胞病毒(RSV)、单纯疱疹病毒(HSV-1)、柯萨奇病毒(COX-B5)和手足口病毒(EV71)的抑制作用。结果甲醇冷浸提取物抗病毒效果较好,对COX-B5的TI值为16.476,最大抑制率为88%,与阳性对照药利巴韦林(TI值为17.482,最大抑制率为85%)相差不大。结论鸡骨草冷浸提取物具有体外抗RSV、HSV-1、COX-B5的活性。

Objective To investigate the antiviral activity of cold - soak extraction ofAbrus cantoniensis Hance. Methods Four dif- ferent strains of viruses, including RSV, HSV - 1 ,COX - B5 and EV71 were used for in - vitro antivirus experiment, and the antiviral ac- tivity of Abrus cantoniensis Hance were evaluated by the cytopathic effect （CPE） method and CCK -8. Thepapeutie indexes were calculated by the Reed -Muench method. Antivirus stability experiments of Abrus cantoniensis Hance were carried out at the same time. Results The TI of Methanol cold - soak extraction and Ribavirin were 16. 476 and 17. 482. It showed Methanol cold - soak extraction was as effec- tive as the Ribavirin. Conclution It indicated that cold - soak extraction of Abrus cantoniensis Hance have antiviral activity against RSV, HSV - 1 and COX - B5