EV71感染手足口病患儿血清炎性细胞因子及免疫球蛋白水平的变化及意义

Changes and Significance of Serum Levels of Inflammatory Cytokines and Immunoglobulin in Children with Hand-foot-and-mouth Disease Infected by EV71

目的观察肠道病毒71型(EV71)感染手足口病(HFMD)患儿血清细胞因子及免疫球蛋白水平变化,探讨炎症及免疫学机制在手足口病发病中的意义。方法选择2015年6月~2016年8月收治的65例EV71感染HFMD患儿为观察组,65例健康儿童为对照组。酶联免疫吸附试验法(ELISA法)检测血清中IL-6、IL-8、TNF-α水平,免疫散射比浊法检测血清IgA、IgG、IgM水平。结果与对照组相比,观察组血清IL-6、1L-8及TNF-α水平显著升高(t值分别为7.94、6.87、9.56,P<0.01),血清免疫球蛋白IgA、IgG及Ig M水平也有上升,差异有统计学意义(t值分别为6.71、7.96、8.39,P<0.01)。结论炎性细胞因子和免疫失衡在手足口病中的发生、发展中发挥重要作用,观察IL-6、IL-8、TNF-α、IgA、IgG及IgM水平的动态变化对判定HFMD病情和疾病防治具有一定的临床应用价值。

Objective Observe the changes of cytokine and immunoglobulin in children with Hand - foot - and - mouth disease （HFMD） infected by Enterovirus 71 （ EV71 ） ,to explore the significance of inflammation and immunological mechanisms. Methods A total of 65 cases of children with HFMD by EVT1 were taken as the observation group,and 65 cases of healthy children were as the control group in June 2015 - August 2016. The serum levels of IL - 6, IL - 8, TNF - α were detected by enzyme - linked immunosorbent assay （ELISA） ,and the levels of IgA,IgG,IgM were detected by immune scattering turbidimetric. Results Compared with control group, the levels of IL - 6, IL - 8 and TNF - α in observation group were significantly increased （ t = 7.94,6.87,9.56, P 〈 0. O1 ） , the levels of IgA, IgG and IgM also increased, differences were statistically significant （ t = 6.71,7.96,8.39,P 〈 0.01 ）. Conclusion The inflammato- ry cytokines and immune imbalance in the development of HFMD play an important role, observe the dynamic changes of IL - 6, IL - 8, TNF - α,IgA,IgG and IgM levels has certain clinical application value in preventing and judging the state of HFMD.