载药纳米粒明胶纤维缓释支架对牙周膜细胞MMPs的影响

Drug-loaded Nanoparticles within Gelatin Scaffolds Inhibits MMPs Expression in Periodontal Ligament Cells

目的 :研究载免疫抑制剂SB203580的纳米粒-明胶(Ms-SB203580-gelatin)纤维缓释支架对牙周膜细胞(human periodontal ligament cells,h PDLCs)的基质金属蛋白酶(matrix metalloproteninases,MMPs)表达的影响。方法 :通过透析法制备载药纳米胶束(Micelles-SB203580,Ms-SB203580),并通过电纺法构建载纳米粒明胶支架,检测支架材料的细胞相容性和对牙周膜细胞MMP-2、MMP-13基因和蛋白的表达影响。结果:透射电镜显示载药纳米粒能负载于明胶纤维中;细胞与支架复合后增殖情况良好;实验组MMP-2、MMP-13的表达低于阳性对照组。结论:载药纳米粒明胶纤维缓释支架能明显地抑制炎症因子,可进一步应用于牙槽骨骨组织工程。

Objective： The study aimed to evaluate whether the SB203580-1oaded nanoparticles-gelatin （Ms-SB203580- gelatin） scaffolds are related to reduction of matrix metalloproteninases（MMPs） expression in human periodontal ligament cells （hPDLCs）. Methods： The drug-loaded micelles （Micelles-SB203580, Ms-SB203580）was obtained by dialysis bag method and fabricate drug-loaded gelatin scaffolds by electrospining. Cell compatibility and the gene as well as protein ex- pression of MMP-2,MMP-13 were measured. Results： The gelatin scaffolds contain drug-loaded micelles were proved by TEM, The hPDLCs seeded at the scaffolds demonstrated a good biocompatibility. MMP-2 and MMP-13 expression were stain slightly in the experimental group than that of the positive group. Conclusion： The results suggest that Ms- SB203580-gelatin scaffolds have a potential to obviously reduce inflammation and may be effective for alveolar bone repair in the future.