FZD1在肾癌中表达及其临床意义

Expression and clinical significance of FZD1 in renal cell carcinoma

目的检测FZD1在配对肾癌及癌旁组织,肾癌细胞系与正常细胞系中m RNA表达水平,探讨其表达与临床病理特征之间关系。方法运用RT-PCR荧光定量方法,从基因水平检测FZD1在52对肾癌组织和癌旁组织,肾癌细胞株(ACHN)和正常人肾近端小管上皮细胞株(HK-2)中表达量差异。结果 FZD1的表达量与患者年龄、性别及病理分型无关(P>0.05),但与肿瘤直径、AJCC临床分期、Fuhrman分级、淋巴结转移、远处转移密切相关(P<0.05)。FZD1在肾癌组织和肾癌细胞中表达均上调,差异有统计学意义(P<0.05)。结论 FZD1在肾癌中表达明显升高,且与不良预后有关,可能作为肾癌临床预后生物标志物之一。

Objective To detect mRNA expression level in renal cell carcinoma cell line and normal cell line in FZD1 matching renal cancer and adjacent tissues to explore relationship between its expression and clinical pathological characteristics. Methods Fuorescence quantitative RT-PCR method was used to test expression level of FZD1 in renal carcinoma cell line （ACHN） and proximal renal tubular epithelial cell line （HK-2） of normal people in 52 couples of renal cell carcinoma tissues and adjacent tissues. Results Expression level of FZD1 was not related to age,gender and pathological type of patients（P 〉 0.05）,but it was closely related to tumor size,AJCC clinical stage,Fuhrman grade,lymph node metastasis and distant metastasis （P 〈 0.05）.Expression levels of FZD1 in renal cell carcinoma tissues and renal carcinoma cell were both improved（P 〈 0.05）.The difference was statistical significant. Conclusion Expression level of FZD1 in renal cell carcinoma is significantly improved and it is related to poor prognosis,which can be regarded as one of biomarkers of clinical prognosis of renal cell carcinoma.