基于转录组数据的月季SSR标记开发及花色遗传性分析

Development of SSR Markers Based on the Transcriptome Data of Rose and Analysis of Flower Color Inheritance

在转录组数据的基础上,以Rosa laxa(疏花蔷薇)、R.rugosa‘White’(‘白玫瑰’)、R.cv.‘Pink Peace’(‘粉和平’)、R.multiflora var.albo-plena(白玉堂)、R.xanthina Lindl f.normalis(单瓣黄刺玫)5个亲缘关系相差比较远的种或品种为筛选和开发SSR引物的植物材料,基于所开发引物对杂交组合‘电子表’ב红法兰西’所得的79株F_1代群体进行了遗传多样性评价。结果表明:1)设计SSR引物80对,筛选出具有目的条带的SSR引物44对,具有多态性的SSR引物28对。2)对‘电子表’ב红法兰西’的F1代群体的遗传多样性进行评价,杂交F1代群体的遗传距离在0.52~0.94之间。3)聚类结果与后代的花色分离有一定相关性,当遗传距离为0.52时,F1代群体聚为2大类,其中一类跟母本‘电子表’(HT-13)聚为一类,花色大多是黄色;另一类跟父本‘红法兰西’(HT-12)聚在一起,花色普遍为红色到粉色之间。研究认为,这一花色分离成因主要与花色遗传有关,在这方面红色对黄色为显性性状;这一分离情况可能也与色素黄酮与花色素苷等色素代谢途径有关。

On the basis of the transcriptomic data of rose,the SSR primers were developed with plant materials of R. laxa,R, rugosa ＇White＇,R. cv. ＇Pink Peace＇ ,R. multi flora Thunb. var. albo-plena and R. xanthina Lindl f. normalis, which had far relationship between each other. The genetic diversity of F1 group hybridized from R. cv. ＇Funkuhr＇ ×R. cv. ＇Red France＇ was evaluated by using these developed primers. The results were as follows. 1）80 pairs of SSR primers had designed, among which 44 were with target fragment,besides,28 were with polymorphism. 2）The genetic distance of F1 group was between 0.52 and 0.94. 3） Clustering analysis showed that F1 group at genetic distance 0.52 was divided into two categories,one was with their female parent ＇Funkuhr＇ （HT-13） ,in which the flower color was generally yellow,the other was with male parent ＇Red France＇ （HT-12）,in which the color was generally between red and pink. The results suggested that the cause of the color separation was mainly associated with color inheritance, that the color red was the dominant character to yellow. The color separation might also associated with metabolism of flavonoids and anthocyanin.