紫玉米苞叶花色苷与牛血清白蛋白相互作用研究

Study on the Interaction between Purple Corn Bract Anthocyanins and Bovine Serum Albumin

采用荧光光谱法、紫外可见吸收光谱法、高效液相色谱法研究紫玉米苞叶花色苷与牛血清白蛋白(BSA)之间的相互作用。结果表明,紫玉米苞叶花色苷与BSA之间存在一定的相互作用,结合程度随着紫玉米苞叶花色苷浓度的增加而增加,二者结合后,会引起BSA结构的变化。紫玉米苞叶中6种主要的花色苷成分与BSA结合程度不同,其结合能力大小依次为:天竺葵-3-(6'-丙二酰葡萄糖苷)>芍药-3-(6'-丙二酰葡萄糖苷)>天竺葵-3-葡萄糖苷>矢车菊-3-葡萄糖苷>矢车菊-3-(6'-丙二酰葡萄糖苷)>芍药-3-葡萄糖苷。

Fluorescence spectrometry, UV-VIS absorption spectroscopy, and high-performance liquid chro- matography （HPLC） were used to research the interaction between purple corn bract anthocyanins and bovine serum albumin （BSA）. The results showed that there was a relationship on the purple corn anthocyanins bract and BSA. The bonding degree increased with the increase of the concentration of anthocyanins . Furthermore, the structure of BSA was changed after the combination. The binding abilities of six anthocyanins in purple corn bract was different. The order of combining ability was as follows- pelargonidin-3-（6j -malonylglucoside）〉pe- onidin-3-（6＇-malonylglucoside）〉pelargonidin-3-goucoside〉cyanidin-3-glucoside〉cyanidin-3-（6＇-malnyyl- glucoside）〉peonidin-3-glucoside.