木贼有效部位抑制氧化低密度脂蛋白致内皮细胞凋亡及Caspase-3的表达

Study on Effective Part of Equisetum to Inhibit Endothelial Cell Apoptosis Induced by Oxidized Low Density Lipoprotein and Expression of Caspase-3

目的探讨木贼有效部位抑制氧化低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞(HUVEC-ECV304)凋亡的可能机制。方法体外ox-LDL诱导人脐静脉内皮细胞损伤,用木贼有效部位进行干预;采用流式细胞术检测细胞凋亡率及Caspase-3蛋白;反转录聚合酶链反应(RT-PCR)检测内皮细胞Caspase-3mRNA表达。结果 ox-LDL可明显诱导内皮细胞凋亡,上调Caspase-3mRNA及Caspase-3蛋白表达,木贼有效部位干预后细胞凋亡率明显下降(P<0.05),Caspase-3mRNA及Caspase-3蛋白表达明显下调(P<0.01)。结论木贼有效部位通过对凋亡相关基因的调控最终抑制Caspase-3蛋白的表达来减少内皮细胞的凋亡。

Objective ： To study the possible mechanism of inhibiting human umbilical vein endothelial ceils （ HUVEC - ECV304） apoptosis induced by oxidized low density lipoprotein （ ox - LDL）. Methods ： Ox - LDL in vitro was used to induce human umbilical vein endothelial cell injury and effective part of Equisetum was used to intervene. Flow cytometry was used to detect cell apoptotie rate and Caspase - 3 protein and reverse transcriptase polymerase chain reaction （ RT - PCR） was used to detect endothelial cell Caspase - 3 mRNA expression. Results ： Ox - LDL could induce the apoptosis of endothelial cells and up - regulate Caspase - 3 mRNA and Caspase - 3 protein expression. The apoptotic rate significantly decreased （P 〈 0. 05 ） after the intervention of the effective part of Equisetum and Caspase - 3 mRNA and Caspase - 3 protein expression obviously down - regulated （ P 〈 0. 01 ）. Conclusion ： The effective part of Equisetum can inhibit the apoptosis of endothelial cells by controlling the expression of Caspase - 3 protein through regulating apoptosis - related genes.