喜马拉雅旱獭鼠疫自然疫源地牧羊犬耶尔森菌感染和携带状况初步调查

Yersinia infection and carrier in shepherd dog on the natural foci of Marmota himalayana plague

目的了解喜马拉雅旱獭鼠疫自然疫源地牧羊犬耶尔森菌感染和携带状况,探讨3种致病性耶尔森菌在自然界是否存在替代或拮抗关系,为鼠疫防控提供新方法。方法采集喜马拉雅旱獭鼠疫疫源地牧羊犬血清、肛拭子标本进行致病性耶尔森菌的检测与分析。结果检测阿克塞县、肃北县、肃南县、天祝县牧羊犬血清分别为88、94、70、64份,阳性率分别为31.82%(28/88)、32.98%(31/94)、2.86%(2/70)、1.56%(1/64)。采集阿克塞县、肃北县、肃南县、天祝县的牧羊犬肛拭子共171份进行致病性耶尔森菌分离,动物间鼠疫流行猛烈的阿克塞、肃北分别分离出1株克氏耶尔森菌和2株非致病性小肠结肠炎耶尔森菌。结论喜马拉雅旱獭鼠疫自然疫源地动物间鼠疫流行猛烈地区的牧羊犬感染和携带非致病性小肠结肠炎耶尔森菌。

We studied Yersiniainfection and carriage in shepherd dog on the natural foci of Marmota himalayana plague and investigated the substitution or antagonism relationship among three pathogenic Yersiniain nature,providing a novel approach for prevention and control of plague.Blood sample and rectal swab specimens from shepherd dogs were collected.Rectal swab specimens were detected and isolated for Y.enterocoliticanand Y.pseudotuberculosis.IHA was used to measure the positive rate of F1 antibody.Results showed that among 88,94,70 and 64serum specimens respectively from Akesai,Subei,Sunan,and Tianzhu,IHA positive rates were 31.82%,32.98%,2.86% and 1.56% separately.A total of 236 anal swab specimens of shepherd dog were collected,among which one Y.kristensenii strain and 2non-pathogenic Y.enterocoliticastrain were recovered from Akesai and Subei respectively,where plague was violently prevalent in animals.All the results indicate that shepherd dogs in natural foci of M.himalayana plague where plague in animals are prevalent with non-pathogenic Y.enterocolitica,is an evidence of antagonism relationship in three pathogenic Yersinia.