山东生猪屠宰环节沙门氏菌血清型及耐药性测试

Resistance and serotype of Salmonella isolated from pig slaughtering process in Shandong Province

目的了解山东省生猪屠宰感染沙门氏菌耐药状况,指导临床用药。方法利用沙门氏菌血清型快速分型试剂盒鉴定血清型,微量肉汤稀释法对298株沙门氏菌进行8大类13种药物的药敏试验。结果鉴定出9种血清型,以德尔卑、鼠伤寒沙门氏菌为主;298株沙门氏菌对13种药物的耐药状况不同,对四环素类药物多西环素、四环素的耐药率较高,分别为97.99%(292/298)、80.20%(239/298),对粘杆菌素E最为敏感,耐药率仅为2.01%(6/298),其次为奥格门丁和氧氟沙星,耐药率分别为2.35%(7/298)和4.03%(12/298),多重耐药率为81.88%(244/298),优势耐药谱型为四环素-多西环素。结论山东省生猪屠宰环节沙门菌优势血清型为德尔卑沙门氏菌,对不同药物的耐药情况不同,不同屠宰环节的耐药性存在显著差异,多重耐药现象严重,耐药谱型种类繁多。

We investigated serotype and resistance of Salmonella during pig slaughtering in Shandong Province,China,providing basic data for the risk assessment of Salmonellaand for guiding the clinical medication.We used rapid classification kit to identify Salmonellaserotype,adopted broth microdilution method to detect the resistance of 13 kinds drugs belong to 8categories.Result showed that the identified 9kinds of serotype were mainly S.derbyand S.typhimurium.The resistance to13 kinds drugs of 298 Salmonella were different.The higher percentage of tetracycline drugs as Doxycycline（DOX）and Tetracycline（TE）were 97.99% and 80.20%,respectively,which was most sensitive to Colistine E.The resistant rate was only2.01%,following by Amoxicillin/Clavulanic acid and Ofloxacin which were 2.35%and 4.03%,and the multiple resistant rate was 81.88%.TE-DOX was the regnant drug-resistant spectrum.In conclusion,the predominant serotype of Salmonellain links of pig slaughtering in Shandong Province is S.derby,and resistance is different to the different drugs.The drug resistance of different slaughter links exist significant differences,multiple drug resistance is serious,and drug-resistant spectrum are varied.