基于双颜色传感器的真菌霉素快速检测方法

A Rapid Detection Method of Mycotoxins Using Double Color Sensors

针对生物学检测真菌霉素的过程比较复杂、成本高、不易操作等问题,提出了一种利用真菌霉素类物质溶液颜色直接测量溶液浓度的快速检测方法,设计了一个双颜色传感器检测系统.通过颜色传感器测得溶液颜色信息来快速确定被检测物质的质量分数并实现颜色信息采集过程中的动态白平衡,使颜色检测对环境光的变化具有自适应性.最后建立样品溶液颜色的三原色(RGB)值与浓度之间的线性关系.实验结果表明,样品数据的相对标准偏差(RSD)低于5%,利用该快速检测方法建立的线性关系计算得到的溶液浓度与样品实际浓度的相对误差(RE)低于7%,满足实验室精密度和准确度的要求.同时,与传统检测方法相比,该方法快速、方便,并降低了检测成本.

To improve the detection efficiency and reduce the testing cost in available mycotoxins detection methods, a rapid detection method was proposed and a color detection system was designed. In this detection system, two color sensors were applied, one was for the measurement of the color information in the sample solution, the other was for the measurement of the white balance parameters of the ambient light in real time to realize the dynamic white balance and make the color detection system be adaptive to the changing ambient light. Finally, the linear relationship between the mycotoxins concentration and the RGB values of the sample solution was established. The experimental results show that, the relative standard deviation（RSD） of the sampled data is less than 5%, and the relative error（RE） of the concentration calculated by the linear relationship and the actual concentration of the sample is less than 7%, both of which meet the laboratory requirements of precision and accuracy respectively. Compared with the common detection methods, this method is fast, convenient, and reduces the detection cost as well.