紫花菜中花青苷的分离纯化与组分分析研究

Isolation and component analysis on anthocyanins from purplebroccoli

以紫花菜花青苷为原料,采用D101、HDP450、HDP600和AB-8四种大孔吸附树脂对花青苷的吸附与解析特性进行了比较研究,并在此基础上,采用最佳大孔树脂对花青苷纯化过程中的静态、动态吸附和解析附条件进行了优化研究。结果表明AB-8大孔树脂具有较好的吸附和解析能力,是纯化紫花菜花青苷的最佳树脂,花青苷的最佳纯化工艺参数为:上样样品浓度为0.027mg/m L,洗脱液为70%乙醇,洗脱液p H为1。利用UPLC-MS/MS法对紫菜花提取液中花青苷进行定性分析,结果表明,紫菜花中可能有矢车菊素-3-槐糖苷、芍药素-3,5-双葡萄糖苷2种花青苷,为紫菜花的综合应用提供技术参考和理论依据。

Purple broccoli anthocyanin was used as raw material, D101, HDP450, HDP600 and AB-8 four kinds of macroporous adsorption resinswere used to study the adsorption and desorption characteristicsof anthocyanin.The dynamic adsorption by macroporous resin was optimized. The results showed that AB-8 macroporous resin had better adsorption and desorption ability, and was the best resin for purification of purple broccolianthocyanin. The optimum purification technologywere ： sample concentration 0.027mg/mL, elution liquid 70% ethanol, eluent pH value 1. A quantitative analysis of anthocyanins in purple broccoli by ultraperformance liquid chromatography tandem triple- quadrupole mass spectrometry （UPLC-MS/MS） was developed. The results showed that the purple broccoli may have cyanidin 3- sophoroside, paeonidin 3, 5-diglucoside two kinds of anthocyanins.The study provided the technical and theoretical references for the comprehensive usage of purple broccoli.