丹参酮对肝癌细胞在体内外生长的抑制作用

Inhibition on the growth of tanshinone on hepatic carcinoma in vitro and in vivo

目的:探讨丹参酮在细胞水平和动物体内对肝癌细胞的抑制作用。方法:采用体外抑瘤实验培养人肝癌细胞Bel-7402,通过MTT法检测其体外抑瘤率;体内实验采用肝癌H22移植瘤法,以抑瘤率、胸腺指数和脾指数为指标,探讨丹参酮的抑瘤作用。结果:细胞实验表明,丹参酮在100μg/m L时抑瘤效果最佳,抑瘤率为(81.95±1.33)%。丹参酮在40、80、120 mg/kg时,对H22实体瘤的抑制率分别为29.70%、45.09%和49.13%。丹参酮在120 mg/kg时小鼠的脾指数和胸腺指数明显高于模型组(P<0.05)。结论:丹参酮对肝癌细胞在体内外均有一定的抑制作用。

Objective ： To study the effect of Tanshinone on hepatic carcinoma in vitro and in vivo. Methods： After being treated with Tanshinone, the inhibitory rate of Tanshinone to BEL-7402 human hepatocarcinoma cells were examined by MTT assay. The model of noumenal transplantation tumor of H22 were established, using anti-tumor rate, thymus index and spleen index to estimate the effect of tumor suppression of Tanshinone. Result： The results of cell experiment showed that the optimal concentration of Tanshinone suppressing the growth of hepatoma cells was 100 μg/mL, and the tumor inhibiting rate was （81.95 ± 1.33） %. The rate of inhibition were 29.70%, 45.09% and 49.13% respectively when the mice were administrated with Tanshinone of 40, 80 and 120 mg/kg. The thymus index and spleen index of mice in group of 120 mg/kg Tanshinone were higher than that of model group （P 〈 0.05, respectively） . Conclusion ： Tanshinone could inhibit the growth of hepatocarcinoma in vitro and in vivo.