分光光度法测定水力空化对绿原酸的影响

Determination of the effect of hydrodynamic cavitation on chlorogenic acid by spectrophotometry

为探明水力空化对绿原酸的影响,通过空化装置产生空化效应并作用于绿原酸溶液。研究了不同入口压力、温度、作用时间等条件下水力空化对绿原酸的影响。以分光光度法监测水力空化处理绿原酸溶液中其含量及色度,采用紫外分光光度法在吸收波长为324 nm时对溶液中绿原酸定量分析,同时采用可见分光光度法在吸收波长为560 nm时对绿原酸溶液进行色度测定。结果发现,水力空化对绿原酸的影响明显,随着压力增大、温度升高或作用时间延长,溶液中绿原酸的含量减小且溶液色泽加深。这为绿原酸在食品和药品等行业中的应用做出了贡献,也为水力空化在制糖工艺、废水处理等工业化中的应用提供了重要的参考。

In order to find out the influence of hydrodynamic cavitation on the chlorogenic acid, the cavitation effect was produced by the cavitation device and the effect on the solution of chlorogenic acid. The effects of different inlet pressure, temperature and time on the effects of hydraulic cavitation on the chlorogenic acid were studied. Based on the spectrophotometric monitoring of hydraulic cavitation treatment chlorogenic acid solution and its content and color by utraviolet spectrophotometry in the absorption wavelength of 324 nm quantitative analysis of chlorogenic acid in solution, while using visible spectrophotometry in the absorption wavelength of 560 nm for chlorogenic acid colorimetric determination. It was found that the influence of the hydrodynamic cavitation on the chlorogenic acid was obvious. The content of chlorogenic acid in solution decreasing and the color of solution deepening with the temperature increasing, the time growing and the pressure rising. It has contributed to the application of chlorogenic acid in food and pharmaceutical industries. It provides an important reference for the application of hydraulic cavitation in the sugar process, wastewater treatment in industrialization.