抗人IgG_4单克隆抗体用于ELISA法诊断血吸虫病与疗效考核的研究

Antihuman IgG_4 monoclonal antibody used in ELISA for diagnosis and cura-tive effect assessment of Schis tosomiasis.

目的探讨检测特异性IgG4诊断血吸虫病的效果及疗效考核价值。方法采用SEA间接ELISA法检测血吸虫病人血清中的特异性IgG和IgG4抗体及对肺吸虫、肝吸虫病人和健康人血清的交叉反应以及血吸虫病人治疗后不同时期特异性IgG和IgG4抗体的变化。结果血吸虫病人血清中IgG4敏感性为93.02%,特异性为100%,而IgG抗体的敏感性为95.35%,特异性为97.62%,IgG4与IgG无论其敏感性还是其特异性差异均无显著性(P>0.05),与其它寄生虫病的交叉反应,IgG4显著低于IgG且差异显著(P<0.05),血吸虫病人治后6个月IgG4抗体阴转率显著高于IgG(P<0.05),治后12个月IgG4的阴转率与IgG比较差异非常显著(P<0.01)。结论ELISA法检测特异性IgG的诊断效能与IgG4相似,而特异性IgG4抗体可能系一短程抗体,用于评价疗效更具实际应用价值。

Objective To explore the value of IgG 4 -specific in patient's serum for the assessment of curative effect and diagnosis of Schistosomiasis.Method The IgG-specific and IgG 4 -specific antibodies in sera of patient s with Schistosomiasis,and the cross-reaction with people having Clonorchiasis or Paragonimiasis or healthy people,the changes in IgG-specific and IgG 4 -specific antibodies at different times during treatment were detected by indirect ELISA.Result s Detecting Schistosomiasis patient serum,the sensitivities of IgG 4 and IgG were93.02%and95.35%,and their specificities were100%and97.62%respectively,neither sensitivity nor specificity had significant differ-ence(P>0.05).But about the cross-reaction with other parasitic diseases,IgG 4 was signifi-cantly lower than IgG(P<0.05).Six months after the patients had been treated,the con-version rate of IgG 4 positive individuals was significantly higher than of IgG(P<0.05),twelve months after treatment,there was very significant difference between the two groups (P<0.01).Conclusion The value of IgG 4 is similar to that of IgG in dignosis of Schistosomiasis,but since IgG 4 -specific antibody is a short-term antibody,it might be more practically valuable in the assessment of curative effect of Schistosomiasis chemotherapy