基于转录组的沙棘木蠹蛾简单重复序列特征分析

Feature analysis of simple sequence repeats in Eogystia hippophaecolus transcriptome

为开发沙棘木蠹蛾微卫星信息,利用已获得的转录组数据,对其EST-SSR位点进行发掘,进而分析其特征。结果发现含SSR的序列5126条,识别的SSR总数为7499个,SSR出现频率为51.41%。微卫星序列主要以单碱基重复为主,发生频率为39.52%。研究共发现77种碱基重复基元,所占比例最高的为(A/T)n(73.74%),其次是(AT/AT)n(3.37%)。微卫星多为重复次数为10且长度为10 bp的短序列。研究结果为沙棘木蠹蛾的SSR分子标记研究,遗传多样性分析,种群遗传结构以及关键性状基因的发掘等研究奠定基础。

In order to exploit SSR information of Eogystia hippophaecolus,we identify EST-SSR loci and analyze their features according to the transcriptome of E. hippophaecolus. Results show 5126 unigenes contain 7499 SSR loci in total（ 51. 41%）. Mononucleotide repeats predominated with an occurrence frequency of 39. 52%. There are 77 kinds of repeat motifs existing in E. hippophaecolus transcriptome.（A/T）n（73. 74%） is most frequent in all the repeat types,and next is（AT/AT）n（3. 37%）. Most of the SSR are less than ten times of repetition and are 10 bp in length. The results should contribute to researches in SSR marker, genetic diversity, population genetic structure and genomic signatures identification in E. hippophaecolus.