摘要
目的探讨丝氨酸/苏氨酸蛋白激酶AKT(the serine/threonine kinase,also known as protein kinase B,PKB)、己糖激酶2(hexokinase 2,HK2)在喉鳞状细胞癌组织及癌旁组织中的表达,分析其与喉癌发病诸病理因素间的相互关系。方法采用免疫组化Streptavidian-perosidase(SP)法检测30例喉癌患者的癌组织中AKT和HK2蛋白的表达情况,同期选取其癌旁组织作为对照。结果 AKT、HK2蛋白在喉癌组织中高表达,与肿瘤的分化程度和有无淋巴结转移密切相关,差异具有统计学意义(P<0.05);AKT、HK2蛋白的阳性表达与患者的年龄、肿瘤发生部位以及临床分期无关(P>0.05);喉鳞癌组织中AKT与HK2的表达呈正相关(r=0.424,P=0.019)。结论喉鳞癌组织中AKT、HK2蛋白高表达与肿瘤的侵袭和转移密切相关,可提示预后不良。
Objective To explore the expressions of serine/threonine kinase AKT/PKB and hexokinase-2 ( HK2 ) in laryngeal squamous cell carcinoma, and study the relationship between their expressions and all pathological factors of this tumor. Methods Immunohistochemieal Streptavidian-perosidase (SP) method was used to examine the expressions of AKT and HK2 in 30 specimens of laryngeal squamous cell carcinoma and 30 of para-carcinoma tissue. Results The expressions of AKT and HK2 in carcinoma specimens were higher than those in para-careinoma tissues, and their expressions were closely related with differentiation and lymphatic metastasis of the tumor ( all P 〈 0.05 ). The expressions of AKT and HK2 were not related to patients' age, primary position and clinical stage of the tumor ( all P 〉 0.05 ). Spearman' s rank correlation analysis showed that the expression of AKT was positively correlated with that of HK2 ( r = 0. 424, P = 0. 019 ). Conclusion In laryngeal squamous cell carcinoma, the expressions of AKT and HK2 are significantly related with invasion and metastasis, which prompts poor prognosis of the tumor.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2017年第3期196-201,206,共7页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
河北省自然科学基金(2016206535)
河北省医学科学研究重点课题计划(20160120)
关键词
AKT
HK2
免疫组化
喉鳞癌
糖酵解
AKT
HK2
Immunohistochemistry
Laryngeal squamous cell carcinoma
Glycolysis