AKT和HK2在喉鳞状细胞癌中的表达及意义

Expressions and clinical significances of AKT and HK2 in laryngeal squamous cell carcinoma

目的探讨丝氨酸/苏氨酸蛋白激酶AKT(the serine/threonine kinase,also known as protein kinase B,PKB)、己糖激酶2(hexokinase 2,HK2)在喉鳞状细胞癌组织及癌旁组织中的表达,分析其与喉癌发病诸病理因素间的相互关系。方法采用免疫组化Streptavidian-perosidase(SP)法检测30例喉癌患者的癌组织中AKT和HK2蛋白的表达情况,同期选取其癌旁组织作为对照。结果 AKT、HK2蛋白在喉癌组织中高表达,与肿瘤的分化程度和有无淋巴结转移密切相关,差异具有统计学意义(P<0.05);AKT、HK2蛋白的阳性表达与患者的年龄、肿瘤发生部位以及临床分期无关(P>0.05);喉鳞癌组织中AKT与HK2的表达呈正相关(r=0.424,P=0.019)。结论喉鳞癌组织中AKT、HK2蛋白高表达与肿瘤的侵袭和转移密切相关,可提示预后不良。

Objective To explore the expressions of serine/threonine kinase AKT/PKB and hexokinase-2 （ HK2 ） in laryngeal squamous cell carcinoma, and study the relationship between their expressions and all pathological factors of this tumor. Methods Immunohistochemieal Streptavidian-perosidase （SP） method was used to examine the expressions of AKT and HK2 in 30 specimens of laryngeal squamous cell carcinoma and 30 of para-carcinoma tissue. Results The expressions of AKT and HK2 in carcinoma specimens were higher than those in para-careinoma tissues, and their expressions were closely related with differentiation and lymphatic metastasis of the tumor （ all P 〈 0.05 ）. The expressions of AKT and HK2 were not related to patients＇ age, primary position and clinical stage of the tumor （ all P 〉 0.05 ）. Spearman＇ s rank correlation analysis showed that the expression of AKT was positively correlated with that of HK2 （ r = 0. 424, P = 0. 019 ）. Conclusion In laryngeal squamous cell carcinoma, the expressions of AKT and HK2 are significantly related with invasion and metastasis, which prompts poor prognosis of the tumor.