人乳头状瘤病毒和p53基因及H-ras基因在喉癌细胞中的表达

Express of human papillomavirus, p53 and H-ras gene in laryngeal cancer

目的 :研究喉癌细胞 p5 3基因、H - ras基因突变及其与人乳头状瘤病毒 (HPV)感染的关系 ,探讨其在喉癌发生发展中可能起的作用。方法 :采用分子生物学技术 ,包括聚合酶链反应、单链构型多态性分析、限制性内切酶长度多态性分析 ,及 DNA序列分析等方法 ,对 2 8例喉癌进行人乳头状瘤病毒、p5 3基因及 H - ras基因检测。结果 :2 8例喉癌中有 5 0 %在 p5 3基因的第 7～ 8外显子有突变 ,其中 4例行 DNA序列分析表明均有碱基替换、插入或丢失 ;而 H- ras基因第 12密码子的突变率仅 3.5 7% ;高危型 HPV(HPV- 16或 - 18)感染在喉癌细胞中有一定的特异性 ,且与 p5 3基因突变有一定的相关性。结论 :在喉癌的发生发展过程中高危型 HPV感染和 p5 3基因突变起着重要的作用 ,且两者之间可能存在某种内在的联系。

Objective: To study the abnormality of p53 and H ras gene in laryngeal cancer cells and their relation to the infection of human papillomavirus (HPV) subtypes. Methods: The HPV subtypes, p53 and H ras gene were examined in 28 cases of laryngeal cancer by polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP), restriction fragment length polymorphism (RFLP) and DNA sequencing. Results: p53 gene mutation existed in 50% cases of laryngeal cancer and DNA sequencing showed base substitution, insertion or deletion in 4 cases; The positive rate of the H ras oncogene mutation was only 3.57%; All high risk HPV infections(HPV 16 or 18) were distinctly seen in the cancer cases, especially in cases with p53 mutation. Conclusion: The high risk HPV subtype infection and p53 gene abnormality may play an important role in laryngeal cancer progression and these two factors may be correlated to each other.