肺癌纤维支气管镜刷落细胞中p16基因外显子2丢失的研究

Deletion of p16 gene exon 2 in bronchofibroscopic brush-off cells of human lung carcinoma

目的 :探讨 p16基因外显子 2丢失与原发性肺癌发生发展之间的相关性 ,以及纤维支气管镜刷落细胞中检测 p16基因外显子 2丢失代替手术标本检测的可行性。方法 :在纤维支气管镜毛刷脱落细胞中采用 PCR-电泳-紫外成像条带密度扫描法 ,以β- actin基因为内对照 ,检测及分析病理证实的原发性肺癌患者病灶侧与其相对正常侧 p16基因外显子 2丢失的情况。结果 :p16基因外显子 2丢失率在肺癌患者相对正常侧毛刷脱落细胞中为 0 ( 0 /19 ) ,在病灶侧毛刷脱落细胞中为 35.5% ( 11/31) ,两者比较有统计学显著差异 ( P<0 .0 1)。小细胞肺癌 ( SCLC)标本中丢失率为 0 ( 0 /7) ,非小细胞肺癌 ( N SCLC)标本中丢失率为 50 % ( 11/2 2 ) ,两者比较有统计学显著差异 ( P<0 .0 5)。结论 :p16基因外显子 2丢失可能与 N SCLC的发生发展相关。纤维支气管镜直视下病灶处毛刷脱落细胞检测 p16基因外显子 2丢失率可代替手术标本以协助术前诊断与治疗。

Objective: To analyze the relationship between deletion of p16 gene exon 2 and lung cancer and to evaluate the possibility of detecting p16 gene exon 2 deletion in brush off cell instead of resected lung cancer mass. Methods: p16 gene exon 2 deletion in bronchofibroscopic brush off cells of lesion side and corresponding normal side was detected through PCR electrophoresis Image. Image value of p16 Vs β actin ≤0.5 was considered as p16 gene exon 2 deletion. Results: The rate of p16 gene exon 2 deletion in normal side was 0(0/19), whereas in lesion side was 35.5%(11/31), there is a significant statistical difference( P <0.01). In SCLC(small cell lung carcinoma) samples, the rate of p16 gene exon 2 deletion was 0(0/7), whereas in NSCLC(non small cell lung carcinoma) samples, that was 50%(11/12)( P <0.05). Conclusions: p16 gene exon 2 deletion might be related to the oncogenesis and development of lung carcinoma, especially NSCLC. Brush off cell specimens may replace surgical specimens in Detecting p16 gene exon 2 deletion.