用人乳腺癌细胞增殖法检测化学物的雌激素样作用

Assessment of the estrogenicity of endosulfan and other chemicals with the in vitro proliferation of human breast cancer cell

目的 :引入一种外源性雌激素的快速筛检方法 ,检测外源性化学物的雌激素样活性。方法 :用人乳腺癌细胞体外增殖法 ( E- SCREEN) ,检测硫丹等化学物的雌激素样活性。结果 :成功地建立了 E- SCREEN法 ,该法可检测到 1× 10 - 13 m ol/ L的雌二醇。发现在该试验条件下 ,己烯雌酚为雌激素受体的全激动剂 ,硫丹为雌激素受体的部分激动剂 ,而 4 -羟化他莫昔酚无雌激素样作用。结论 :E- SCREEN法敏感性高 ,并有快速、简便等优点 ,适用于大量化合物的初筛。

Objective: To develop a rapid screening method for xenoestrogens and to screen the estrogenicity of some environmental chemicals. Methods: The E SCREEN test was developed based on proliferation of human breast cancer cells (MCF 7), and the estrogenicity of diethylstilbestrol, 4 hydrotamoxifen and endosulfan was assessed. Results: The E SCREEN detected estradiol at very low concentration as 1×10 -13 mol/L. It was found that diethylstilbestrol was a full agonist of estrogen receptor, endosulfan was a partial agonist, while 4 hydrotamoxifen lacked estrogenic effects at this assay. Conclusion: The E SCREEN test is sensitive, rapid, easy to perform and, therefore, suitable for large scale screening for estrogenicity of environmental chemicals.