负载纳米磁铁颗粒的PLGA微球的可控制备研究

Preparation and Characterization of Fe_3O_4 Magnetic Nanopaticles Loaded PLGA Microspheres

目的采用微流控法制备聚乳酸-羟基乙酸共聚物(PLGA)微球,并同时负载纳米磁铁颗粒,为利用PLGA复合微球实现靶向给药,以及应用MRI进行追踪提供实验基础。方法设计微流控三相装置,用微流控法制备得到PLGAFe_3O_4复合微球,并对所得微球的形貌及Fe_3O_4纳米磁铁颗粒在微球内部的分布情况进行分析测试。结果微流控法制备的PLGA-Fe_3O_4复合微球粒径均一,形貌和分散性好。元素分析结果证实了Fe元素的存在。TEM观察表明,Fe_3O_4纳米磁铁颗粒在微球内部以纳米尺径存在,且分散良好,未出现团聚现象。结论证实了利用微流控法制备负载纳米磁铁颗粒PLGA微球的可行性,为PLGA微球提供了进一步功能化以及靶向给药和MRI追踪的可能性。

Objective To prepare Fe3O4 magnetic nanopaticles loaded poly（lactic-co-glycolic acid） （PLGA） microspheres using microfluidics, and to characterize this mierospheres for future possible applications as targeted drug delivery and MRI tracking. Methods PLGA-Fe3O4 microspheres were prepared by three-phase microfluidic devices. Morphology of microspheres and distribution of Fe3O4 magnetic nanopatieles inside PLGA microspheres were analyzed. Results The PLGA-Fe3O4 microspheres prepared by microfluidic method had uniform particle size distribution and excellent dispersibility. The results of elemental analysis confirmed the presence of Fe, and the results of TEM observation showed that Fe3O4 magnetic nanopatieles were well dispersed inside the microspheres without agglomeration. Conclusion The feasibility of Fe3O4 magnetic nanopaticles loaded PLGA microspheres prepared by microfluidie method is demonstrated. The current study thus provides possibility of using PLGA microspheres for targeted drug delivery and MRI tracking.