呋喃唑酮人工抗原的制备

Preparation of Furazolidone Artificial Antigens

[目的]制备呋喃唑酮人工抗原。[方法]通过重氮化法和戊二醛法将半抗原FZ与载体蛋白质牛血清白蛋白(BSA)和卵清蛋白(OVA)耦联,制备人工免疫抗原和检测抗原,经SDS-PAGE电泳鉴定人工抗原是否耦联成功,用紫外可见分光光度计法测定耦联蛋白浓度。[结果]SDS-PAGE结果显示,用2种方法制备的人工抗原FZ-BSA和BSA、FZ-OVA和OVA的2组蛋白条带迁移距离均发生了变化,耦联物的迁移距离均小于其相对应的载体蛋白。[结论]通过SDS-PAGE鉴定呋喃唑酮人工抗原制备成功。

[ Objective ]To prepare the furazolidone artificial antigens. [ Methods ] Furazolidone （hapte） was conjugated with the carrier proteins of bovine serum albumin （BSA） and ovalbumin （OVA） to prepare artificial antigens by diazotization and glutaraldehyde methods. The prepared artificial antigens were tested by the SDS-PAGE electrophoresis, and the concentration of the coupling protein was determined by UV-visible spectrophotometer. [Results]The SDS-PAGE results showed that the migration distance for two groups of protein bands of FZ-BSA and FZ-OVA with BSA and OVA, which were the synthesized artificial antigens prepared by two kinds of methods, were changed, and the migration distance of couplings were less than their corresponding carrier proteins. [ Conclusion ]The furazolidone artificial antigens are successfully prepared.