荞麦花叶发酵提取物对2型糖尿病db/db小鼠心肌损伤的影响

Effects of extract from fermented buckwheat flower and leaf(EFBFL) on myocardial injury in type 2 diabetic db/db mice

目的探讨荞麦花叶发酵提取物(EFBFL)对自发肥胖2型糖尿病db/db小鼠心肌损伤的保护作用及其可能的机制。方法 9周龄♂db/db小鼠随机分为EFBFL高(EFBFL-H,0.1 g·kg^(-1))、低(EFBFL-L,0.05 g·kg^(-1))剂量组,二甲双胍对照组,模型对照组,每组10只。同时以10只db/m小鼠为正常对照组。各组均每天灌胃给药1次,连续8周。于给药前及给药后第2、4、6、8周末检测小鼠随机血糖(RBG),8周后处死取血清检测肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)和糖基化终末代谢产物(AGEs)含量;HE染色光镜下观察心肌组织形态学改变,电镜下观察心肌超微结构;免疫组化法和Western blot法检测心肌组织葡萄糖转运蛋白4(Glut4)表达。结果 EFBFL能有效抑制db/db小鼠心肌细胞损伤及心肌纤维化的病理进程,降低小鼠血糖及血清中CK、CK-MB、AGEs的含量,增加小鼠心肌组织Glut4蛋白表达水平。结论 EFBFL对自发肥胖2型糖尿病db/db小鼠心肌损伤具有抑制作用,其机制可能与降低小鼠血糖、抑制AGEs的产生及促进心肌细胞Glut4蛋白的表达有关。

Aim To discuss the effectsof extract from fermented buckwheat flower and leaf（EFBFL） on myocardial injury in spontaneously obese type Ⅱ diabetic db/db mice and its mechanism.Methods 9-week-old male db/db mice were randomly divided into high level EFBFL dose group（EFBFL-H,0.1 g · kg^-1）,low level EFBFL dose group（EFBFL-L,0.05 g ·kg^-1）,metformin hydrochloridecontrol group,model control group,and normal control group,with 10 mice in each group.All groups were treated with 8 wks of drugs by gastric perfusion.The random blood glucose（RBG）was tested respectively at the end of 2nd,4th,6th,and 8th wk.Finally,the levels of creatine kinase（CK） creatine kinase MB（CK-MB）,andadvanced glycosylation endproducts（AGEs） were detected after 8wks.The morphological changes of myocardium were observed under light microscope by HE staining,and the ultrastructure of myocardium was observed under electron microscope.Immunohistochemical method and Western blot were used to detect myocardial tissue glucose transporter-4（Glut-4）.Results EFBFLcould repress patho-proceeding of myocardial fibrosis efficiently,and significantly decrease the level of blood glucose,CK,CK-MB,and AGEs in db/db mice.Meanwhile,it could increase the expression of Glut-4 in myocardial tissues of mice.Conclusions EFBFL can prevent myocardial injury in spontaneously obese typeⅡ diabetic db/db mice.The possible mechanism may be related to lowering the level of blood glucose and serum AGEs and up-regulating Glut4 of cardiac muscle.