出芽短梗霉OF-01菌株的筛选及其在烟草香料中的应用

Screening of Aureobasidium pullulans OF-01 and its application in tobacco flavor

用废弃烟叶作原料,通过生香菌筛选和致香成分提取,制备了基于微生物发酵处理的衍香香料,将其与烟叶浸膏进行化学成分与感官比较,结果表明:1)从65株生香细菌和17株生香酵母菌中得到纯系生香细菌11株,生香酵母菌2株,通过风格特征评价最终筛选出出芽短梗霉(Aureobasidium pullulans)OF-01菌株,用于烟叶发酵制备衍香香料;2)采用OF-01菌株发酵烟叶,对发酵后的烟叶碎片进行提取、浓缩,制备烟叶衍香香料,与常规烟叶浸膏相比,其所含酸类(586.18%)、醛类(308.12%)、酚类(235.04%)等致香成分增幅明显,其中苯甲醛、3-羟基-2-丁酮、茄那士酮等致香成分含量增加超过1000%;3)OF-01衍香香料具有明显提调烟香、醇和烟气、降低刺激性、掩盖杂气和改善卷烟吸味的效果,且与云产卷烟的配伍性较好,可作为香料在卷烟生产中使用.

Using discarded tobacco leaves as raw materials,through aroma-bacterial screening and aroma components extracting,tobacco endogenous derived flavor was prepared and comparison was made on chemical components and sensory with tobacco leaf extract. Result indicated that： 1） 11 pure aroma-bacteria and 2 pure aroma-yeast were screened from 65 aroma-bacteria and 17 aroma-yeast. An Aureobasidium pullulans OF-01 was gotten by the style character evaluation eventually,and used for tobacco leaf flavor preparation; 2） A tobacco derived flavor was prepared by OF-01 strains through fermenting extracting,and concentrating. By comparison of the tobacco water extract,acids（ 586. 18%）,phenols（ 235. 04%）,and the alcoholes aldehydes（ 308. 12%） in tobacco derived flavor promote greatly,and the quantities of scholar ketone,3-hydroxy-2-butanone,and benzaldehyde incense increased over 1000%; 3） The tobacco derived flavor could increase armor,smooth smoke,reduce irritation,hide offensive and improve the effects of cigarette smoking. It had better compatibility with Yunnan cigarette,and could be used as flavor in cigarette production.