利普司他汀生产菌株发酵工艺的研究

Study on the Fermentation Process of Strain Which Can Produce Lipstatin

目的研究利普司他汀发酵配方和培养条件,确定最佳生产工艺。方法从利普司他汀的摇瓶发酵着手,通过考察p H、碳源、氮源、无机盐、种龄、接种量、温度、发酵时间等因素的影响,优化发酵培养基以及发酵培养条件。结果最佳初始p H为6.5~7.0;最佳的碳源是丙三醇;最佳的氮源是去脂黄豆粉;对发酵影响最大的无极离子为锌离子;最佳培养温度是30℃;最佳种龄是25 h;最佳接种量是4%~6%。结论采用优化的发酵配方和发酵条件可提高发酵活性单位。

OBJECTIVE To find a best production process by studying Lipstatin fermentation formula and culture conditions. METHODS During the aerobic fermentation, p H, carbon source, nitrogen source, inorganic salt, inoculate age, inoculate quantity, temperature and fermentation time were studied to optimize the fermentation media and culture conditions. RESULTS The most suitable p H for original culture was 6.5-7.0; the best carbon source was glycerol; the best nitrogen source was skimmed bean flour; and zinc ion was mostly impact the fermentation process among the inorganic salt; the best culture temperature was 30 ℃; the suitable fermentation time was 25 h and the best inoculate quantity was 4%-6%. CONCLUSION Fermentation activity unit can be increased through optimized fermentation formula and culture condition.