甘肃紫斑牡丹花粉中脂肪酸的分析研究

Analysis of Fatty Acids in Paeonia Rockii Pollen from Gansu Province

目的分析评价甘肃紫斑牡丹花粉中的脂肪酸类成分。方法采用溶剂法超声提取紫斑牡丹花粉中脂肪酸类成分,经三氟化硼-甲醇法催化甲酯化,以GC-MS-SIM对紫斑牡丹花粉及其破壁花粉样品进行分析。采用HP-5MS石英毛细管色谱柱(30 m×0.25 mm,0.25μm);载气:高纯氦气;程序升温:起始温度50℃,以8℃·min^(-1)升至155℃,保持2 min;再以10℃·min^(-1)升至175℃,保持2 min;以1.3℃·min^(-1)升至190℃,保持8 min;最后以6℃·min-1升至280℃,保持5 min。不分流进样;进样口温度:260℃;进样量:1μL。结果紫斑牡丹花粉中主要含有C8~C24的18种脂肪酸,其中辛酸、肉豆蔻酸、棕榈酸、油酸、亚油酸、γ-亚麻酸含量较高;紫斑牡丹花粉中的脂肪酸以不饱和脂肪酸为主,不饱和脂肪酸占脂肪酸总量的50.45%~73.55%;破壁的紫斑牡丹花粉中脂肪酸总量是未破壁样品的6~7倍。结论所建立的分析方法简便、准确,适于甘肃紫斑牡丹花粉中脂肪酸类成分的分析。

OBJECTIVE To analyze and evaluate the fatty acids in Paeonia rockii pollen from Gansu province. METHODS The fatty acids in Paeonia rockii pollen collected from Gansu province and it＇s wall-broken pollen samples were extracted by ultrasonics method with cyclohexane, then methyl esterificated via BF3-methanol method, and finally detected by GC-MS-SIM. HP-5MS capillary column（30 m×0.25 mm, 0.25 μm） was adopted, and the heating program was 50 ℃programmed to 155 at 8℃ ℃·min^（-1） and kept for 2 min, then increased to 175 at 10℃ ℃·min^（-1） and kept for 2 min, and raised to 190 ℃at 1.3 ℃·min^（-1） and kept for 8 min, finally increased to 280 ℃ at 6 ℃·min^（-1） and kept for 5 min. The sample inlet temperature was set at 260 ℃. The injection volume was 1 μL with splitless mode. High purity helium was used as the carrier gas. RESULTS Paeonia rockii pollen contained 18 kinds of fatty acids（C8-C24）. The content of caprylic acid, myristic acid, palmitic acid, linoleic acid, oleic acid and γ-linolenic acid were the more higher content among 18 kinds of fatty acids. The fatty acids in Paeonia rockii pollen were comprised of polyunsaturated fatty acids which takes up 50.45%-73.55% of the total fatty acids. The wall-broken pollen was almost 6-7 times as amounts of fatty acids as pollen. CONCLUSION The method is simple, accurate, which can be used for fatty acids analysis in Paeonia rockii pollen.