白细胞介素28B基因多态性与慢性HCV感染及抗HCV疗效的关系

Association between interleukin 28B gene polymorphisms and chronic hepatitis C virus infection and response to anti-HCV treatment

目的探讨白细胞介素28B(interleukin 28B,IL-28B)基因单核苷酸多态性(single nucleotide polymorphisms,SNPs)与丙型肝炎病毒(hepatitis C virus,HCV)慢性感染及抗病毒疗效的关系。方法选择河北省慢性丙型肝炎及代偿期丙型肝炎肝硬化患者253例,健康体检者83例作为对照组。TaqMan探针法检测IL-28B rs8099917位点SNPs,分析HCV感染患者与对照组IL-28Brs8099917位点等位基因及基因型分布差异,并探讨患者IL-28Brs8099917位点SNPs与抗HCV疗效的关系。结果患者IL-28Brs8099917位点G、T等位基因分布以及GG/GT、TT基因型分布与对照组比较,差异无统计学意义(P>0.05)。IL-28Brs8099917位点SNPs与患者基线HCV RNA水平及HCV基因型均不相关(P>0.05)。获得完全早期病毒学应答(complete early virological response,cEVR)及持续病毒学应答(sustained virological response,SVR)的患者IL-28Brs8099917位点T等位基因、TT基因型携带率均显著高于未获得cEVR及SVR患者。结论河北省人群IL-28Brs8099917位点SNPs与HCV慢性感染易感性不相关,且与患者基线HCV RNA水平及感染HCV基因型不相关。携带TT基因型患者更易获得cEVR及SVR。

Objective To explore the association of interleukin 28B（IL-28B） single nucleotide polymorphisms（SNPs） with chronic hepatitis C virus（HCV） infection and response to anti-HCV treatment.Methods Two hundred and fifty-three patients with chronic hepatitis C and compensated cirrhosis, and 83 healthy controls were genotyped for the IL-28B rs8099917 polymorphisms by TaqMan system.The relation of IL-28B rs8099917 SNPs and chronic HCV infection, and the association of IL-28B rs8099917 SNPs and virological responses were analyzed.Chi-square test was performed for comparison of categorical data.Results The patients with chronic HCV infection and the healthy controls showed similar frequencies of G, T allele and GG/GT, TT genotypes（P〉0.05）.IL-28B rs8099917 SNPs were neither significantly associated with baseline HCV RNA level nor with HCV genotype（P〉0.05）.The presences of IL-28B rs8099917 T allele and TT genotype were significantly higher in patients achieved early virological response and sustained virological response than non-response patients.Conclusion In Hebei province, polymorphisms in IL-28B rs8099917 were not associated with susceptibility to chronic HCV infection.IL-28B rs8099917 T allele and TT genotype were predictors for better virological responses.