IL-1β与IL-18在急性肺损伤中的表达及临床意义

Clinical significance of IL-1β and IL-18 in acute lung injury

目的基于细胞焦亡理论研究白介素(IL)-1β与IL-18在急性肺损伤(ALI)中的表达及临床意义。方法选取2016年1月至2017年1月在广州中医药大学第一附属医院重症医学科收治的符合ALI患者100例为观察组,期间同时招募50例健康体检者为对照组。酶联免疫法检测并比较两组受试者入组后IL-1β及IL-18的血清浓度水平,并比较观察组中存活患者与病死患者血清IL-1β与IL-18的含量。结果观察组患者经常规治疗后,病死率为38.00%(38/100),而存活率为62.00%(62/100);入组后,两组受试者血清IL-18[(106.43±21.73)pg·mL^(-1)vs(174.29±36.31)pg·mL^(-1)]与IL-1β[(62.14±15.88)pg·mL^(-1)vs(94.35±16.23)pg·mL^(-1)]水平比较,对照组均明显低于观察组,差异均有统计学意义(P<0.05);在临床结局方面,病死组和存活组患者的IL-1β[(118.32±19.52)pg·mL^(-1)vs(87.86±16.49)pg·mL^(-1)]与IL-18[(195.64±36.13)pg·mL^(-1)vs(136.74±23.09)pg·m L-1]水平比较,病死组均明显高于存活组,差异均有统计学意义(P<0.05)。结论细胞焦亡是ALI患者的重要病理机制之一,ALI的发生发展和IL-1β与IL-18的参与有关。

Objective To investigate the clinical significance of interleukin （IL）-1β and IL-18 on the acute lung injury （ALI） based on the mechanism ofpyroptosis. Methods One hundred patients with ALI from the First Affiliated Hospital of Guangzhou University of TCM during January 2016 to January 2017 were treated as the observation group, and 50 healthy volunteers were recruited as the control group. The serum concentrations of IL-1β and IL-18 were tested and compared between the two groups, which were also compared between the survival subgroup （patients that survived） and dead subgroup （patients that died of illness events） in the observation group. Results With the conventional medications, the fatality rate was 38.00% （38/100） in the observation group, while the survival rate was 62.00% （62/100）. The serum concentrations of IL-18 and IL-1β were （106.43±21.73） pg·mL^-1 and （62.14＋15.88） pg·mL^-1 in the control group, as compared with （174.29±36.31） pg·mL^-1 and （94.35±16.23） pg·mL^-1 in the observation group （P〈0.05）. Moreover, the serum concentrations of IL-1β and IL-18 were （118.32±19.52） pg·mL^-1 and （195.64±36.13） pg·mL^-1 in the dead subgroup, as compared with （87.86±16.49） pg·mL^-1 and （136.74±23.09） pg·mL^-1 in the survival subgroup （P〈 0.05）. Conclusion Pyroptosis was one of the significant mechanisms of ALI, and the occurrence and development of ALI were related to the participation of IL-1β and IL- 18.