高压氧预处理对大鼠缺血再灌注皮瓣基质金属蛋白酶-2和基质金属蛋白酶-9及缝隙连接蛋白43表达的影响

Effect of hyperbaric oxygen preconditioning on expression of MMP-2, MMP-9 and Cx43 in rat abdominal skin flap model of ischemia-reperfusion injury

目的 探讨高压氧预处理对大鼠腹部皮瓣缺血再灌注损伤过程中基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）以及缝隙连接蛋白43 （Cx43）表达水平的影响.方法 选取成年雄性SD大鼠18只,体重220～250 g,采用随机数表法,随机分为假手术（SH组）、缺血再灌注（IR组）和高压氧预处理（HBO组）.HBO组在术前3d,每天行2次常规高压氧治疗,每次间隔12h.3组大鼠均行以右侧腹壁浅动脉为蒂的皮瓣成形术,IR组和HBO组大鼠加行皮瓣缺血3h.术后3d取样行MMP-2、MMP-9以及Cx43免疫组织化学染色和Western Blot分析.结果 HBO组Cx43表达量明显高于IR组（15.03±3.66比36.01±4.12）,MMP-2和MMP-9表达量明显低于IR组（MMP-2：12.01±1.23比5.98±1.48;MMP9：16.77±2.01比11.48±1.77）.结论 高压氧预处理可减少大鼠腹部皮瓣缺血再灌注损伤过程中MMP-2和MMP-9的表达,并提高Cx43的表达.

Objective To study the effect of hyperbaric oxygen preconditioning management on the expression of MMP-2,MMP-9 and Cx43 in a rat abdominal skin flap model of ischemia-reperfusion injury.Methods Eighteen male adult SD rats,weight ranged from 220-250 g,were randomly divided into three groups：sham group （SH）,ischemia-reperfusion group （IR） and hyperbaric oxygen preconditioning group （HBO）.All the rats in HBO group received hyperbaric oxygen treatment twice each day,apart 12 hours,during the last three days before operation.Abdominal skin flap with superficial epigastric artery as pedicle was established.In HBO and IR group,3 hours of ischemia was performed.On the 3rd postoperative day,samples were taken to assess the expression of MMP-9,MMP-2 and Cx43 by immunohistochemical staining and Western blot.Results Compared with IR group,the expression of Cx43 was significantly increased （IR 15.03±3.66;HBO 36.01±4.12） and the ex pression of MMP-9 and MMP-2 was decreased （MMP 2：IR 12.01±1.23;HBO 5.98±1.48;MMP9：IR 16.77±2.01;HBO 11.48±1.77）.Conclusions Hyperbaric oxygen preconditioning for the rat abdominal skin flap model of ischemia-reperfusion injury has inhibitory effect on the expression of MMP-9 and MMP-2,and stimulative effect on the expression of Cx43.