摘要
目的 体外研究miRNA-16（miR-16）在乳腺癌患者中的表达及其对乳腺癌细胞增殖、迁移能力的影响.方法 采用荧光定量聚合酶链反应（PCR）技术检测30例乳腺癌组织中miR-16的表达情况.将miR-16模拟物转染到乳腺癌细胞系MDA-MB-231和MCF-7中,通过CCK-8实验和Transwell实验分别检测miR-16对MDA-MB-231和MCF-7细胞增殖和迁移能力的影响.结果 在30例乳腺癌组织标本中,23例（76.7%）miR-16表达下调.转染miR-16模拟物后,MDA-MB-231和MCF-7细胞的增殖和迁移能力受到明显抑制.结论 miR-16在乳腺癌患者中低表达,miR-16在体外可明显抑制肿瘤细胞的增殖和迁移能力.
Objective To investigate the expression of miRNA-16 （miR-16） in breast cancer patients and its effect on the proliferation and migration of breast cancer cells. Methods Polymerase chain reaction （PCR） was used to detect the expression of miR-16 in 30 breast cancer patients. miR-16 mimics was transfected to MDA-MB-231 and MCF-7 cell, and CCK-8 as well as Transwell assay was applied to detect the effect of miR-16 on cell proliferation and migration of MDA-MB-231 and MCF-7 cells. Results Among 30 breast cancer patients, the expression of miR-16 was down-regulated in 23 cases. Cell proliferation and migration of MDA-MB-231 and MCF-7 cells were inhibited significantly after transfection of miR-16 mimics. Conclusion miR-16 is down-regulated in breast cancer patients. miR-16 inhibits significantly the cell proliferation and migration of breast cancer cells in vitro.
出处
《肿瘤研究与临床》
CAS
2017年第6期366-369,共4页
Cancer Research and Clinic
基金
国家自然科学基金（81402205）
山西省自然科学基金（2014011037-1）
