2-羟基戊二酸对胶质母细胞瘤细胞增殖和迁移的影响

Effect of 2-hydroxyglutarate on cell proliferation and migration of human glioblastoma cells

目的探讨2-羟基戊二酸(2-HG)对异柠檬酸脱氢酶(IDH)野生型胶质母细胞瘤增殖、迁移的作用。方法通过细胞培养,细胞增殖实验(CCK-8)检测不同浓度(5、10、15、20、30、40 mmol/L)2-HG对胶质瘤细胞的增殖抑制作用,细胞迁移实验检测细胞迁移能力,聚合酶链式反应(PCR)检测相关基因mRNA的表达。结果外源性加入2-HG使胶质母细胞瘤细胞形态发生间质样改变,随浓度的升高作用愈明显,呈浓度依赖性,促进胶质母细胞瘤细胞的增殖和迁移,下调上皮细胞标志物E-钙黏蛋白(E-Cadherin)的表达,上调间质细胞标志物波形蛋白(Vimentin)和β-链蛋白(β-catenin)的表达。结论 2-HG能够促进IDH野生型胶质母细胞瘤的增殖、迁移。

Objective To investigate the effect of 2 - hydroxyglutarate （2 - HG） on proliferation and migration of isocitrate dehydrogenase （IDH） wild - type glioma. Methods The wild - type glioma cells were cultured. CCK8 method was used to detect the inhibiting effects of different concentrations of 2 - HG （5, 10, 15, 20, 30, 40 retool/L） on the proliferation of glioma ceils. Cell migration test was perforned to detect the migration of glioma cells. And PCR was carried out to detect the expression of mRNA of related genes. Results Exogenous treatment of 2 - HG transformed the morphol- ogy of glioblastoma cells in concentration - dependent manner. Exogenous treatment of 2 - HG promoted the abilities of proliferation and migration, down -regulated the epithelial cell marker E -cadherin, and up -regulated the mesenchymal cell marker vimentin and β - catenin. Conclusion 2 - HG can promote the proliferation and migration of IDH wild - type glioblastoma cells.