摘要
目的评价血浆抗可溶性肝抗原抗体(抗SLA)检测在乙型肝炎病毒(HBV)相关慢加急肝衰竭(HA)患者中的意义。方法使用抗SLA酶联免疫检测试剂盒,检测H-A患者(H-A组)、肝硬化(LC)患者(LC组)、慢性乙型肝炎(CHB)患者(CHB组)和健康对照者(HC组)血浆抗SLA,分析不同临床组间抗SLA的表达差异,以及与肝功能相关指标的关系。结果慢性HBV感染(CHB+LC+H-A)组的抗SLA水平均显著高于HC组(P<0.001),而H-A组抗SLA的OD值显著高于CHB组(P<0.001)、LC组(P<0.001);在HBV感染者中,抗SLA的OD值水平与ALT(r=0.260,P=0.046)及TBIL(r=0.646,P<0.001)水平呈正相关,与HBV-DNA无明显相关性(r=-0.069,P=0.598)。在H-A组,抗SLA的OD值水平与总胆汁酸呈正相关(r=0.478,P=0.024),与凝血酶原活动度、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、直接胆红素(DBIL)、总胆红素(TBIL)无显著相关性。结论这种检测方法可用于评估不同慢性HBV感染者抗SLA的表达差异,H-A患者高表达抗SLA可能与肝细胞的严重损伤及免疫发病机制相关。
Objective To evaluate the significance of serum anti -SLA level in patients with HBV -related acute - on - chronic liver failure （ H - A）. Methods A total of 26 patients with H - A （ H - A group）, 16 patients with chronic hepatitis B （CHB group） , 17 patients with liver cirrhosis （LC group） and 14 healthy controls were enrolled （HC group）. The serum level of anti - SLA was detected by enzyme linked immunosorbent assay. Results The optical density value level of anti - SLA in patients with HBV infection was significantly higher than that of HC group （P 〈 0. 001 ）. Moreover, the level of anti - SLA in H - A group was significantly higher than those in CHB group （P 〈0. 001 ） and LC group （P 〈 O. 001 ）. The level of anti - SLA was positively correlated with ALT （r =0. 260, P =0. 046） and TBIL （r =0. 646, P 〈 0. 001 ）, hut not correlated with HBV - DNA （r = -0. 069, P =0. 598） in patients with HBV infection. In H - A group, the level of anti -SLA was positively correlated with total bile acid （r = 0. 478, P = O. 024）, but not correlated with pro- thrombin activity （PTA）, ALT, AST, DBIL or TBIL. Conclusion It is suggested that the difference of anti - SLA ex- pression among the patients with HBV infection could be assessed using this method, and the high level of anti - SLA in H - A patients might be correlated with the progression and prognosis of acute - on - chronic liver failure.
出处
《广东医学》
CAS
北大核心
2017年第13期1977-1980,共4页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:81270025)
