摘要
目的利用PCR方法鉴定SPF金定鸭的性别。方法根据已有报道合成一对禽类性染色体上的染色质解螺旋蛋白DNA结合蛋白1(CHD1)基因的通用引物gCHD。采集培育中的85只SPF金定鸭血液样品,提取血液基因组DNA,进行PCR扩增。通过分析PCR扩增产物凝胶电泳后的条带数判定禽类性别。结果 PCR扩增产物经琼脂糖电泳分析后,雌性鸭样品扩增出CHD1-Z和CHD1-W,而雄性鸭样品只出现CHD1-Z。结论本文建立的方法可作为禽类性别的有效鉴定方法。
Objective To determine the sex of SPF Jinding Duck, the universal primer gCHD of avian chromosomal DNA-binding protein 1 （CHD1） gene on avian chromosomes were synthesized. Methods Blood of 85 SPF Jinding Duck were collected and genomic DNA was extracted for PCR amplification. The sex of the birds were determined by analyzing the number of bands after gel electrophoresis. Results The CHD1-Z and CHD1-W were found in the PCR products of agarose gel electrophoresis from females and CHD1-Z was from males. Conclusions The PCR can be used as an effective method for sex identification in birds.
出处
《实验动物与比较医学》
CAS
2017年第3期249-251,共3页
Laboratory Animal and Comparative Medicine
基金
国家科技支撑计划(2015BAI07B02-02)
中国农业科学院基本科研业务费专项(Y2016PT41)
黑龙江省自然科学基金重点项目(ZD2016006)
