Gal-1,ANXA2和PRPH在SD大鼠耳蜗核的表达

Expression of Gal-1,ANXA2 and PRPH in cochlear nucleus on SD rats

目的 :采用同位素相对标记和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQi)技术分析耳蜗核(cochlear nucleus,CN)区域特异性膜蛋白。方法 :出生60 d的雄性SD大鼠分为4组,分别是CN、下丘(inferior colliculus,IC)、上橄榄核(superior oliver complex,SOC)、大脑其余部位(Rest)。提取这4个部位组织的细胞膜蛋白,再采用iTRAQi技术检测出CN区域特异性蛋白。通过Uniprot检索这些蛋白的功能和参与的生物代谢过程。结果:本实验最终得到17种CN区域特异性膜蛋白。对CN区域蛋白进行基因本体(gene ontology,GO)分析发现,这些蛋白主要参与生物调节与发育。UNIPROT检索发现3种蛋白与神经系统功能相关,即半乳糖凝集素-1(galectin-1,Gal-1)、膜联蛋白A2(annexin-A2,AXNA2)和外周蛋白(peripherin,PRPH)。Gal-1与神经生长发育相关;AXNA2参与多种生物调节过程;PRPH与神经退行性病变相关。其中,Gal-1可促进轴突生长、调节突触可塑性且具有神经保护作用;AXNA2可能参与了神经递质释放过程,且有神经营养作用;PRPH在高表达时具有神经毒性,和神经丝蛋白共同参与神经退行性病变。结论:Gal-1、ANXA2和PRPH在耳蜗核中高度表达,可能与中枢信息处理相关,这为进一步研究中枢听觉处理障碍发病机制提供了一定的理论基础。

Objective：Using isobaric tags for relative and absolute quantitation （iTRAQ）,to find the region-typical membrane proteins of cochlear nuclues（CN）.Methods：The subjects are male D60 SD rats.The four regions CN,Inferior Colliculus（IC） ,Superior Oliver Com-plex （SOC） and the rest of the brain （Rest） ,were seperated. The region-typical proteins of CN were identified with iTRAQ. The functions of these proteins and the process of biological metabolism involved were discussed seriously through Uniprot.Results： Seventeen region-typical proteins of CN were identified.GO （Gene Ontology） pathway analysis indicates that they are mainly involved in biological regulation processes and development. Gal-1 （Galectin-1）,ANXA2（Annexin-A2）, and PRPH（Peripherin）were found to be related to the function of the nervous system through Uniprot.Gal-1 was involved in neural development and synapase plasticity, and has neuroprotective effects. ANXA2 had neurotrophic effects, and might be involved in the release of neurotransmitters. Highly expressed PRPH led to neurotoxi-city, and was involed in neurodegenerative disease.Conclusion： In CN, Gal-1, ANXA2 and PRPH proteins might be related to central audi-tory processing, and it provides theoretical basis for further researches on the pathogenesis of central auditory processing disorder.