NF-κB和MMP-9在亚临床甲减孕鼠胎盘中的表达及意义

Expression and significance of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism

目的:通过研究核转录因子-κB(Nuclear Kappa B,NF-κB)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在亚临床甲减孕鼠胎盘组织中的变化,分析亚临床甲减孕鼠早期先兆流产的发生机制。方法:采用免疫组织化学法、Western blot检测NF-κB和MMP-9在亚临床甲减孕鼠胎盘组织中(E10)和正常组中的表达情况,分析二者与亚临床甲减孕鼠早期先兆流产的相关性。结果:NF-κB和MMP-9在亚临床甲减孕鼠胎盘组织中的阳性表达率分别为16.7%和50.0%,而在正常组胎盘组织中的阳性率分别为83.3%和100.0%,差异均有统计学意义;Western blot检测结果与免疫组织化学检测结果基本一致。结论:NF-κB和MMP-9在亚临床甲减孕鼠胎盘组织中低表达,导致滋养细胞侵袭力下降,对判断妊娠期亚临床甲减早期先兆流产发生机制具有重要指导意义。

Objective： To explore the expressions of Nuclear Kappa B （NF-κB） and matrix metalloproteinase-9 （MMP-9） in placental tissues of pregnant rats with subclinical hypothyroidism and study the occurrence mechanism of threatened abortion. Methods： The expressions of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism and normal pregnant rats were detected by immunohistochemistry and Western blotting assay. Correlations between the expressions and the occurrence mechanism of threatened abortion of subclinical hypothyroidism were analyzed. Results： The positive rates of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism were 16.7% and 50% ,respectively,while in normal placental tissues were 83.3% and 100% ,respectively.The differences were significant（all P〈0.05）.The results of Western blotting assay were consistent with those of immunohistochemistry. Conclusion： The expression levels of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism have a high correlation with the invasion,and joint detection of the above indicators ,and it is instructional for judgement the occurrence mechanism of threatened abortion of subclinical hypothyroidism.