GC-MS法测定海狗油中不饱和脂肪酸

Determination of Polyunsaturated Fatty Acids in Seal Oil by GC/MS

[目的 ]建立气相色谱 -质谱法 (GC- MS) ,测定海狗油中不饱和脂肪酸。 [方法 ]比较不同类型毛细管柱对样品进行分析 ,选用 PEG- 2 0 M毛细管柱及最佳分析条件 ,用归一化法测定不饱和脂肪酸百分含量 ,并以 GC- MS法对其化学成分进行鉴定。 [结果 ]共分离出 2 5种脂肪酸 ,对其中 3个主要成分进行了定性分析 ,其结果与文献相吻合。 [结论 ]本法简便、快速。

To establish a GC/MS method for the determination of polyunsaturated fatty acids in seal oil. The sample was prepared from seal oil, then the components of fatty acid in it were determined by GC/MS. Their relative contents were measured by normalized method of chromatographic peak areas. 25 kinds of fatty acids were separated from seal oil, of which the main components, Eicosapentaenoic acid(EPA), Docosahexaenoic acid(DHA) and Docosapentaenoic acid(DPA),were analysized qualitatively. The results were the same as the related literatures reported. [CONCLUSION]This method is simple, sensitive and rapid.