阳和汤对自身免疫性甲状腺炎大鼠甲状腺结构及甲状腺自身抗体的影响

Effect of Yanghe decoction on thyroid structure and thyroid autoantibodies of autoimmune thyroiditis rats

目的观察阳和汤对自身免疫性甲状腺炎模型大鼠甲状腺组织结构及甲状腺自身抗体的影响。方法选取Lewis大鼠30只,正常组10只,其余20只大鼠采用高碘饮食联合猪甲状腺球蛋白皮下注射的方法建立自身免疫性甲状腺炎动物模型,造模成功大鼠分为模型组和中药组,干预8周后采用放免法检测大鼠甲状腺功能及甲状腺球蛋白抗体(thyroglobulin antibody,TGAb),采用酶联免疫吸附测定检测甲状腺过氧化物酶抗体(thyroid peroxidase antibody,TPOAb),甲状腺组织石蜡包埋切片,HE染色。结果与正常组相比,模型组及中药组大鼠促甲状腺激素(TSH)明显降低,血清游离甲状腺素(FT_4)及TPOAb、TGAb明显升高,均具有统计学意义(P<0.01);与模型组大鼠相比,中药组大鼠TPOAb、TGAb降低,有统计学意义(P<0.01);中药组大鼠TPOAb治疗前后差值,与正常组及模型组相比均具有明显差异(P<0.01);中药组大鼠甲状腺组织切片与模型组相比,淋巴细胞浸润程度及甲状腺滤泡细胞破坏均减少。结论阳和汤可降低自身免疫性甲状腺炎大鼠甲状腺自身抗体,减少甲状腺组织淋巴细胞浸润程度,保护甲状腺滤泡细胞等作用。

Objective To observe the effects of Yanghe decoction on thyroiditis structure and thyroid autoantibodies of autoimmune thyroiditis rats. Methods 30 Lewis rats were selected,10 rats in the normal group,the remaining 20 rats were fed with high iodine diet combine with subcutaneous injection with porcine thyroglobulin to establish autoimmune thyroiditis model. The successful model rats were divided into model group and traditional Chinese medicine（ TCM） group,after 8 weeks of intervention,the thyroid function and thyroid globulin antibody（ TGAb） were detected by radioimmunoassay（ RIA）,enzyme linked immunosorbent assay（ ELISA） was used to detect thyroid peroxidase antibody（ TPOAb）,thyroid tissue was embedded in paraffin and stained with HE. Results Compared with normal group,TSH of model group and TCM group were significantly decreased while the FT4,TPOAb,TGAb were increased,the results had statistically significant（ P〈0. 01）; compared with model group,TPOAb and TGAb og TCM group was lower,the results had statistical significance（ P〈0. 01）; D-value of TPOAb in before and afterthe treatment of TCM group had obvious difference compared with the normal group and the model group（ P〈0. 01）; compared with the model group,the degree of lymphocyte infiltration and the destruction of thyroid follicle cells were decreased in the thyroid tissue sections of the TCM group. Conclusion Yanghe decoction can reduce thyroid autoantibodies and the degree of thyroid lymphocyte infiltration,and can protect the thyroid follicular cells in autoimmune thyroiditis rats.