补肾益肺消癥方对α-SMA及SP-C蛋白对肺纤维化大鼠病变肺组织分布及表达的影响

Influences of Bushen Yifei Xiaozheng decoction on the distribution and expression of α-SMA and SP-C protein in IPF rat

目的探讨补肾益肺消癥方干预肺纤维化大鼠α-SMA及SP-C蛋白在病变肺组织中分布及表达的作用机制。方法以博来霉素致大鼠肺纤维化的动物模型,以吡非尼酮作为阳性对照药物,补肾益肺消癥方分别于模型过程中、造模后及实验全程进行干预,观察动物活动及体重变化,免疫组化检测病变肺组织中α-SMA、SP-C蛋白表达。结果大鼠体重显示,模型组体重较正常组明显减轻(P<0.05),中药防治组体重较模型组明显升高(P<0.05)。免疫组化、免疫荧光结果显示,模型组病变肺组织中α-SMA、SP-C蛋白表达较正常组明显升高(P<0.05),阳性组和中药组α-SMA、SP-C蛋白表达较模型组明显减低(P<0.05)。结论补肾益肺消癥方可以通过减少α-SMA的表达及成熟SP-C蛋白沉积,减少成纤维灶的形成,抑制内质网应激反应,延缓特发性肺纤维化的病理进程,从而达到治疗的目的。

Objective To investigate the mechanism of Bushen Yifei Xiaozheng decoction in the distribution and expression of α-SMA、SP-C protein of pulmonary fibrosis rats. Methods IPF rat model were induced by Bleomycin. Rats were treated with pirfenidone and Bushen Yifei Xiaozheng decoction respectively before modeling. To observe the changes of activity and the weight of rats during the modeling,after modeling and the whole experiment. The expression of α-SMA and SP-C protein of lung tissue was detected by immunohistochemical staining method. Results The weight of model group was lighter than the normal group（ P〈0. 05）,and the Chinese medicine group gained more weight than the model group（ P〈0. 05）. Compared with the model group,the expression of α-SMA and SP-C were decreased significantly in the treatment group（ P〈0. 05）. Conclusion Bushen Yifei Xiaozheng decoction can reduce the expression of α-SMA and the deposition of the mature SP-C,release the endoplasmic reticulum stress,then to achieve the purpose of treatment.