沙苑子不同规格炮制品中沙苑子苷A的含量比较研究

Comparative study on the content of complanatuside A in different specifications of astragali complanati semen

目的建立沙苑子中沙苑子苷A的含量测定方法,考察沙苑子不同规格炮制品中沙苑子苷A的含量及其变化情况。方法用盐水、黄酒、米醋三种辅料,按照烘、炒、蒸三种不同工艺,炮制出9种不同规格沙苑子炮制品,采用高效液相色谱(HPLC)法测定不同规格沙苑子中沙苑子苷A的含量。结果沙苑子苷A在0.0209μg^0.4180μg范围内线性关系良好(r=0.999 9,n=5);平均加样回收率为97.91%,RSD为1.48%,准确度符合要求。沙苑子生品、盐水闷润炒干品、盐水闷润烘干品、盐水闷润蒸后烘干品、黄酒闷润炒干品、黄酒闷润烘干品、黄酒闷润蒸后烘干品、米醋闷润炒干品、米醋闷润烘干品、米醋闷润蒸后烘干品中沙苑子苷A含量分别为0.98、0.89、0.85、1.16、0.88、0.90、1.09、0.85、0.87、1.18 mg/g。结论建立的HPLC检测方法快速准确、重复性好、专属性强;盐水、黄酒或米醋三种辅料闷润炒干和闷润烘干可导致沙苑子中沙苑子苷A含量下降,而辅料闷润蒸后烘干则使得沙苑子苷A含量增加,尤以米醋闷润蒸后烘干品中沙苑子苷A含量最高。

Objective To establish a method of content determination of complanatuside A in astragali complanati semen,and study the contents and changes of different specifications. Methods Saline water,rice-vinegar,rice-wine was used as excipients,according to the three different processes of baking,frying and steaming,the astragali complanati semen was made up of 9 kinds of products with different specifications,then the content of complanatuside A was determined by HPLC. Results Complanatuside A showed a good linearity relationship in the range of 0. 0209 μg ~ 0. 4180 μg（ r = 0. 999 9,n = 5）. The average recovery was 97. 91%,RSD was 1. 48%,the accuracy met the requirements. The contents of complanatuside A in not-processed product,roasting product after saline water moistening,drying product after saline water moistening,roasting product after saline water moistening and steaming,roasting product after rice-wine moistening,drying product after rice-wine moistening,roasting product after rice-wine moistening and steaming product,roasting product after rice-vinegar moistening,drying product after rice-vinegar moistening and drying product after rice-vinegar moistening and steaming,the content of complanatuside A was 0. 98,0. 89,0. 85,1. 16,0. 88,0. 90,1. 09,0. 85,0. 87,1. 18 mg/g respectively. Conclusion The established HPLC detection method is fast,accurate,repeatable and strong specificity; roasting or drying after moistening with saline water,rice-vinegar or rice-wine can reduce the content of complanatuside A in astragali complanati semen,however the drying after moistening and steaming with excipients can increase the content of complanatoside A,the content of drying product after rice-vinegar moistening and steaming is highest.