肠杆菌科三种主要细菌产超广谱β-内酰胺酶的检测及药敏分析

Detection of extended-spectrum β-lactamases and antibiotic susceptibility test analysis of 3 kinds of main Enterobacteriaceae bacteria

为了解肠杆菌科三种主要产超广谱 β内酰胺酶细菌的产酶率及耐药情况 ,采用双纸片协同法和NCCL推荐的标准纸片扩散法对 117株细菌进行检测 ,检出率分别为肺炎克雷伯氏菌 3 8.1%、大肠埃希氏菌2 8.5 %和肠杆菌属 2 4.3 %。药敏结果显示 ,产酶菌多重耐药现象较普遍 ,产酶株耐药率明显高于非产酶株 ;临床医师和实验室人员应对其有充分认识 ,并掌握本地区和本医院产酶菌的发生及耐药特点 ,对指导临床合理使用抗生素 。

To study the productive rates of extended spectrum β lactamases (ESBLs) and the drug resistance of 3 main Enterobacteriaceae bacteria, 117 strains have been detected with the double disk synergy tests and K B method which is recommended by NCCLs. The productive rate of ESBLs in Klebsiella pneuimoniae was 38.1%, Escherichia coli was 28.5% and Enterobacter was 24.3%. The results of drug resistance tests show that multi drug resistance of ESBLs is general, and the resistance rate of the ESBLs producting strains is much higher than that of the non ESBLs producting ones. Clinical doctors and the people in laboratory should have enough knowledge about it. What′s more, they should master the characters of the production and drug resistance of the ESBLs producting strains in local area and their own hospitals, which has important significance for reasonably using the antibiotic in clinical medicine and controling the epidemic and spread of the ESBLs producting strains.