TNF-α-308基因单核苷酸多态性与乳腺癌易感性的关系

Relationship between single nucleotide polymorphisms of tumor necrosis factor alpha-308 gene and breast cancer susceptibility

目的探讨TNF-α-308基因单核苷酸多态性与乳腺癌易感性的关系。方法采用PCR限制片段长度多态性方法对原发性乳腺癌患者165例和对照组170例健康志愿者进行TNF-α编码基因的308基因位点多态性分析。结果两组TNF-α-308基因多态型和等位基因型分布频率差异无统计学意义(P>0.05)。以有无脉管侵犯、淋巴结转移状况等进行分层分析显示,携带G等位基因的乳腺癌患者脉管侵犯、淋巴结转移风险增高[(OR=2.63,95%CI(1.29~5.37),P=0.01;OR=2.34,95%CI(1.05~5.20),P=0.04]。结论携带G等位基因的女性乳腺癌患者发生脉管侵犯、淋巴结转移的风险较高。

Objective To investigate the relationship between single nucleotide polymorphisms of tumor necrosis factor alpha（TNF-α）-308 gene and breast cancer susceptibility.Methods Polymorphism of TNF-α-308 gene was analyzed with PCR restriction fragment length polymorphism in 165 patients with breast cancer（group A）and 170 controls（group B）.Results There were no significant differences in the distribution frequency of TNF-α-308 polymorphism genotypes and alleles between two groups（P〉0.05）.The stratification analysis showed that higher risks were found in the patients with vascular invasion[OR=2.63,95%CI（1.29-5.37）,P=0.01]and lymphatic metastasis[OR=2.34,95%CI（1.05-5.20）,P=0.04]among breast cancer patients carrying G allele genotype.Conclusion Female breast cancer patients carrying G allele are at higher risk for vascular invasion and lymphatic metastasis.