非洲马铃果果籽提取物的体外抗肿瘤活性

Antitumor Activity of Extracts from Voacanga africana Seeds

分别以不同极性有机溶剂(正己烷、二氯甲烷、乙酸乙酯、丙酮与乙醇)对非洲马铃果的果籽进行索氏提取,测定提取率;以人宫颈癌细胞(Hela Cells)与人肝癌细胞(SMMC-7721细胞株)为对象,对5种有机相的非洲马铃果果籽提取物进行体外细胞毒理性研究,测定提取物抗肿瘤活性。结果表明,马铃果果籽丙酮提取物对SMMC-7721癌细胞具有显著的增殖抑制活性,同时也表现出对Hela癌细胞较高程度的抑制作用;正己烷提取物对Hela癌细胞具有相对较明显的抑制活性,对SMMC-7721癌细胞药理活性不显著;其他有机相提取物几乎没有肿瘤细胞增殖抑制活性。

With different polarity organic solvents （n-hexane, dichloromethane, ethyl acetate, acetone and ethanol）, extraction yield of Voacanga africana seeds by Soxhlet extraction were determinated. The inhibitory activity of extracts against human tumor cell proliferation was tested using Hela Cells and SMMC-7721 Cells as the experimental object and the MTT method as judge standards. The results indicated that V. africana seed extracts of acetone showed excellent inhibitory activity on the proliferation of SMMC-7721 cells and a general inhibition towards Hela cancer cells. And the n-hexane extracts also had relatively significant cytotoxic activity on Hela cancer cells, while the activity towards SMMC-7721 cells was not significant; other organic phase extracts possessed almost no inhibition activity.