鸟巢蕨组培瓶苗培育技术

Cultivation Techniques of Tissue Culture Container Seedlings of Asplenium nidus

对鸟巢蕨Asplenium nidus优良株系孢子离体培养的初代培养、继代培养和生根培养以及移栽等方面进行研究。结果表明,初代培养的最佳培养基是MS+0.1%活性炭,原叶体形成率达80%;在1/3MS+6-BA 1.8mg·L^(-1)+NAA 0.5 mg·L^(-1)增殖培养基中培养,增殖倍数高达5.1;继代芽苗在1/2MS+NAA 0.5 mg·L^(-1)+IBA 0.5mg·L^(-1)生根培养基中,生根率高达95%;移栽的最佳基质为红心土+腐殖土(1:1),移栽成活率达90%。

Primary culture, subculture, rooting culture and transplantation otAsplemum ntaus spores were carried out in this study. Results showed that the best primary culture medium was MS ＋ 0. 1% activated carbon, the formation rate of prothallus was 80%; In the multiplication medium with I/3MS ＋ 6-BA 1.8 mg·L-1 ＋NAA 0.5 mg·L-1, the proliferation times was up to 5.1; in rooting medium with 1/2MS ＋ NAA 0.5 mg·L-1 ＋ IBA 0.5 mg·L-1, the rooting rate of subculture shoot was highest; the best transplanting substrate was the red soil ＋ humus （1 ; 1）, transplanting survival rate was 90%.