三阴乳腺癌细胞受体相互作用蛋白140的表达与癌细胞生长和增殖的相关性

The correlation of RIP140 expression in triple negative breast cancer with the growth and proliferation of cancer cells

目的探讨受体相互作用蛋白140(RIP140)在三阴乳腺癌组织中的表达及其对癌细胞生长和增殖的影响。方法选取2005年2月至2008年2月该院行乳腺癌改良根治手术的患者179例,均能电话随访且术前均未行放射治疗或者化疗。其中,术后诊断三阴乳腺癌41例,非三阴乳腺癌138例,各取癌旁组织10例。采用免疫组织化学法和Western Blot检测3种组织的RIP140表达;慢病毒介导MDA-MB-231细胞RIP140沉默,将RIP140沉默后的MDA-MB-231细胞作为实验组,未沉默的MDA-MB-231细胞作为空白组,分别注入30只裸鼠皮下,以后每周每组各处死10只裸鼠,取皮下肿瘤测量其体积并做石蜡切片,核增殖抗原Ki-67的免疫组织化学法检测皮下肿瘤癌细胞增殖情况。结果 RIP140在三阴乳腺癌组织中的表达明显高于非三阴乳腺癌组织,且均高于癌旁乳腺组织(P<0.05)。RIP140强阳性患者的总生存率低于RIP140表达相对较低的患者(P<0.05)。沉默MDA-MB-231细胞RIP140后,抑制三阴乳腺癌裸鼠皮下肿瘤的生长和增殖。结论 RIP140在三阴乳腺癌组织的高表达与三阴乳腺癌患者预后较差密切相关。沉默三阴乳腺癌细胞RIP140可抑制乳腺癌细胞的生长和增殖。下调三阴乳腺癌细胞RIP140的表达,可抑制三阴乳腺癌的发生和发展。

Objective To explore the expression of RIP140 in tissues of triple negative breast cancers（TNBC） and its effects on the prognosis of triple negative breast cancers patients;To explore the effects on the growth and the proliferation of triple negative breast cancer cells when downregulating of RIP140 in these cells. Methods Retrospective chart reviewed of 179 patients with breast cancer for breast cancer procedures during Feb. 2005 to Feb. 2008 were included. These patients can telephone follow-up,and preoperative were not receiving radiotherapy or chemotherapy. Among of them,41 cases were TNBC and 138 cases were non-TN- BC. Immunohistochemical staining and western blot detected the RIP140 protein expression in these tissues; Lentivirus transfection mediated RIP140 downregulating in MDA-MB-231 cells. 30 BALB/c nude mice were subcutaneous injection of MDA-MB-231 cells, and 10 mice were killed each week. The volumes of the subcutaneous tumors were measured. Immunohistochemical staining of Ki-67 in tumor sections and show the proliferation of MDA-MB-231 cells. Results The expression of RIP140 in TNBC is much higher than non-TNBC tissues;The higher of RIP140 in breast cancer tissues means the worse survival rate of these patients;Lentivirusmediated RIP140 downregulating in MDA-MB-231 cells suppresses the growth and the proliferation of subcutaneous tumors. Con- clusion High expression of RIP140 in TNBC is closely related to the bad prognosis of these patients. Downregulating of RIP140 in breast cancer cells can inhibit the growth and the proliferation of these cells. Above all,this mean downregulating of RIP140 TNBC tissues may as a potential target treatment to TNt3C.