多发性骨髓瘤患者外周血淋巴细胞亚群的变化及临床意义

The clinical significance of lymphocyte subsets in peripheral blood from patients with multiple myeloma

目的探讨初发多发性骨髓瘤(MM)患者淋巴细胞亚群表达水平的变化,分析其免疫功能状态及临床意义。方法收集南京大学医学院附属鼓楼医院初诊为MM的患者20例(患者组),健康体检者25例(健康对照组)。利用流式细胞仪分别检测2组研究对象的外周血淋巴细胞的CD3^+T细胞、CD3^+CD4^+T细胞、CD3^+CD8^+T细胞、B细胞、NK细胞、辅助性T淋巴细胞(Th)1、Th2,以及调节性T细胞(Treg)的表达水平,并进行比较。结果患者组与健康对照组比较,CD3^+T细胞、CD3^+CD4^+T细胞比例的差异无统计学意义(P>0.05);CD4^+/CD8^+的比值、B细胞、Th1、Th2比例降低;CD3^+CD8^+T细胞、Th1/Th2、Treg所占比例比健康对照组明显升高,差异均有统计学意义(P<0.05)。结论 MM患者免疫功能发生紊乱,T细胞亚群的表达异常可能与疾病的发生、发展及预后相关。

Objective To investigatethe lymphocyte subsets from patients with multiple myeloma（MM）, analysis the state of immune function and clinical significance. Methods 21 patients with MM in newly diagnosed and 25 healthy volunteers were re cruitedfrom the Drum Tower hospital affiliated to Medical School of Nanjing University. The differences of CD3 ＋ T cells, CD3＋ CD4＋ T cells,CD3 ＋ CD8＋ T cells, B cells, NK cells, Thl, Th2 and Treg cells in peripheral blood between the patients of MM and healthy people were measured by the flow cytometry. Results Compared with the healthy volunteers and the patients with MM in newly diagnosed, the expression of CD3 - T cell, CD3 ＋ CD4 ＋ , T cell in lymphocyte were no statistically significant （P〉 0.05 ） ; the expression of B ceils, Thl, Th2 cells and the ratio of CD4＋ /CD8＋ in lymphocyte from the patients with MM were lower than healthy control（P〈0.05）;the expression of CD3＋ CD8＋ cells,Treg cells and the ratio of Th1/Th2 were higher than the control group（P〈0.05） ,the differences were statistically significant. Conclusion The immunefunction of patients with multiple myeloma was disorder,the imbalance of T cell subgroup may be associated with the occurrence,development and prognosis of disease.