江苏地产浒苔多糖对四氧嘧啶诱导糖尿病小鼠的降糖作用研究

Effect of Enteromorpha Polysaccharides of Jiangsu Province on Alloxan-Induced Diabetic Mice

目的观察江苏地产浒苔多糖对四氧嘧啶诱导糖尿病小鼠的影响。方法腹腔注射四氧嘧啶建立糖尿病小鼠模型,将小鼠随机分为空白组、模型组、参芪降糖颗粒(500mg/kg)、浒苔多糖低(100mg/kg)、中(200mg/kg)、高(400mg/kg)剂量组,连续灌胃给药28d,观察给药期间动物体质量、进食和饮水量的变化。于给药28d后测定各组小鼠空腹血糖值、口服糖耐量、糖化血清白蛋白以及血清胰岛素的含量。小鼠处死后,剖取胰腺组织进行病理学观察,测定胰岛数和胰岛面积。结果与空白模型组相比,中、高剂量组浒苔多糖能显著降低糖尿病小鼠空腹血糖水平(P<0.01),血糖曲线下面积(P<0.01),显著增多胰岛数目(P<0.01),增大胰岛面积(P<0.01),升高血清胰岛素含量(P<0.05~0.01),且浒苔多糖高剂量组能显著降低模型小鼠糖化白蛋白的浓度(P<0.05)。结论江苏地产浒苔多糖在200~400mg/kg剂量范围内能降低四氧嘧啶诱导糖尿病小鼠的血糖,改善模型小鼠的耐糖力,缓解胰岛病变程度。该结果将为以江苏地产浒苔多糖为原料的功能性保健品的开发提供理论依据。

OBJECTIVE Study the effects of Enteromorpha polysaccharides of Jiangsu province on alloxan-induced diabetic mice was studied. METHODS The diabetic mice were induced by alloxan ip, and treated with distilled water （model group）, Shenqi Jiangtang granule （500 mg/kg）, three different doses of （100, 200, and 400 mg/kg） for 28 days respectively. The changes of body weight, food and water intake were observed during administration. The content of fasting blood glucose, oral glucose tolerance, glycosylated serum albumin and serum insulin levels were measured after 28 days of treatment. The pancre- atic tissues were taken for pathological observation, the islets numbers and islet area were also determined. RESULTS Medi- um and high dose group of can significantly reduce the fasting blood glucose level （P〈0.01）, decrease the area under the curve of blood glucose （P 〈0.01 ）, increase the number of islets and islet area （P 〈0.01 ）, elevate serum insulin level （P 〈0.05 - 0. 01） and high dose of can significantly reduce the content of glycosylated serum albumin （P〈0.05） as compared with the dia- betic model mice. CONCLUSION Within the 200-400 mg/kg dose range of can decrease blood glucose, improve glucose tol- erance and relieve the pancreatic lesion levels of diabetic model mice. The results will provide a theoretical basis for the devel- opment of drugs or functional health care products of Enteromorpha polysaccharides of Jiangsu province