亚低温疗法联合依达拉奉对大鼠脑缺血再灌注损伤的保护作用

Protective effects of mild hypothermia combined with edaravone on rats with cerebral ischemic reperfusion injury

目的探讨亚低温疗法联合依达拉奉对大鼠脑缺血再灌注损伤后的保护作用。方法采用线栓法建立局灶性脑缺血再灌注模型,缺血6h后再灌注,并分为假手术组、模型组、依达拉奉组、亚低温联合依达拉奉组,再灌注24h后进行神经功能缺损评分,采用TTC染色测定脑梗死体积和用免疫组化的方法检测Bax和Bcl-2的阳性细胞数。结果与模型组比较,依达拉奉未能减少脑梗死体积,但能通过下调Bax及上调Bcl-2减少细胞的凋亡,对脑细胞有保护作用;与模型组、依达拉奉组比较,亚低温联合依达拉奉组能通过上调Bcl-2及下调Bax明显降低大鼠神经缺陷评分及减少脑梗死体积。结论亚低温疗法联合依达拉奉对大鼠脑缺血再灌注损伤具有很好的保护作用。

Objective To observe protective effects of mild hypothermia combined with edaravone on rats with cerebral ischemic reperfusion injury.Methods Six hours after the establishment of focal cerebral ischemia by suture method,we performed reperfusion.And 24 hours after successful reperfusion injury,neurological functional deficits in sham-operation group,model group,edaravone group and mild hypothermia combined with edaravone group （combined group） were evaluated.Cerebral ischemic volume was measured by TCC staining and the number of positive Bax and Bcl-2 cells was tested by immunohistochemistry method.Results Compared with model group,edaravone group failed to reduce the infarct volume,but managed to decrease cell apoptosis via down-regulating Bax and up-regulating Bcl-2 for the purpose of protective effect.In addition,compared with model group and edaravone group,the combined group significantly lowered the degree of ischemic reperfusion injury and reduced infarct volume through the up-regulation of Bcl-2 and down-regulation of Bax.Conclusion Mild hypothermia combined with edaravone has good effects on rats with cerebral ischemic reperfusion injury.