丛集性头痛患者炎症细胞因子分析

Analysis of Inflammatory cytokine expression in cluster headache

目的应用抗体芯片分析丛集性头痛患者在头痛急性发作期及发作间期血清中炎性细胞因子的表达变化,探索炎性细胞因子在丛集性头痛发病中可能的作用。方法收集6例丛集性头痛患者急性发作期及发作间期的全血标本离心取血清藏于-80℃冰箱。将生物素做标记的上述12份血清标本,与预先备好的40个主要炎症相关细胞因子特异抗体芯片行膜反应,目标蛋白与红外荧光剂标记的抗链霉生物素抗体结合并曝光,采用红外荧光扫描仪扫描为图像文件后进行分析。结果与发作间期相比,丛集性头痛患者急性发作期血清中多种炎性细胞因子表达水平明显升高,其中炎性因子IL-1β(44.18 vs.68.46)、IL-6(23.08 vs.36.40)、IL-8(151.87vs.328.12)、IL-13(23.93 vs.38.87)、MCP-1(454.80 vs.725.75)及MIP-1β(265.08 vs.515.74)在两组间表达差异有统计学意义(P<0.05)。结论多种炎性细胞因子在丛集性头痛的发病过程中有升高,提示可能有炎症参与丛集性头痛的发病,但具体机制尚需进一步的研究。

Objective To analysis the expression levels of inflammatory cytokines in patients with cluster headache during headache attack period and intermittent period using antibody chips to explore the role of inflammatory cytokines in the pathogenesis of cluster headache. Methods Blood samples from 6 patients with cluster headache were collected during headache attack period and intermittent period. Samples were then centrifugated and stored at-80 degrees refrigerator. Samples were further labeled with biotin and reacted with antibody chips against40 major inflammatory cytokines. The target proteins were conjugated with streptomycin antibody labeled with infrared fluorescent agent, and signals were transformed to images by Licor-odyssey scanner. Results In pairwise comparisons,the levels of some inflammatory cytokines were significantly increased during attacks compared to intermittent period including interleukin-1β（44.18 vs. 68.46, P〈0.05）, interleukin-6（23.08 vs. 36.40, P〈0.05）, interleukin-8（151.87 vs.328.12, P〈0.05）, interleukin-13（23.93 vs. 38.87, P〈0.05）, monoeyte chemoattraetant protein（454.80 vs. 725.75, P〈0.05） and macrophage inflammatory protein-1β（265.08 vs. 515.74, P 〈0.05）. Conclusion Inflammatory cytokines may play an important role in the pathogenesis of cluster headache. However, the mechanism needs further investigation.